The Protein Crystallization Facility (PCF)
The Protein Crystallization Facility (PCF) is an academic, non-commercial, and non-profit research facility, which serves in the first instance the scientific community on the KTH campus and everybody (academia, industry) who has a special and sincere interest in phenomenon of crystallization of protein molecules.
The aim of the PCF is to:
- Quickly respond to the demand of producing protein crystals suitable for structural determination by X-ray crystallography and other medical and industrial applications
- Organize highly specialistic courses on phenomenon of crystallization for medical, scientific and other applications
- Provide tailor-made individual training on phenomenon of protein crystallization
The list of established methodologies and provided services:
- Hanging drop crystallization method
- Sitting drop crystallization method
- Crystallization under oil
- Factorially designed crystallization trials
- Statistical analysis of the crystallization
- Estimating of the precipitation diagram
- Estimating of second virial coefficients
- Production of crystals for structural determination
- Improvement quality of crystals for structural determination
- Screening condition for nucleation (Crystallomic)
- Advanced help for purification of membrane proteins for crystallization
Prices are negotiable individually, for further information please contact Jan Sedzik (email: sedzik@kth.se)
Course 2009 (preliminary date, Autumn 2009)
Title: Molecules - aggregation, nucleation and crystallization beyond medical and other implications.
A short description of the course, and lecture power point notes are available as downloadable files.
Publication
www.worldscibooks.com/lifesci/6930.html
Convener of PCF
Jan Sedzik
Tel : +46-8-790 XXXX
Fax : +46-8-10 52 28
Advisory members
Åke Rasmuson - Royal Institute of Technology, Stockholm
Industrial crystallization
Naomi Chayen - Imperial College, London
Crystallization of proteins
Mitsuo Ataka - AIST, Osaka
Strong magnetic field in crystallization
Hans Hebert - KI, Huddinge
2D-crystallization
Christian Betzel - DESY, Germany
Physico-chemistry of nucleation
