BB1130 Analysis and Purification of Biomolecules 7.0 credits
This course has been discontinued.
Last planned examination: Spring 2019
Decision to discontinue this course:
No information insertedContent and learning outcomes
Course contents
This course is focused on the methods with constitute the foundation for purification and analysis of biological macro molecules, such as nucleic acids and proteins. The techniques which will be discussed often all exist within both biochemical/biotechnological research and industry. During the course these methods will be described and discussed during the lectures, labs and a smaller project. The course book will be complemented with descriptions from other literature and with relevant case studies from research. If the opportunity arises, a visit to a company will be arranged. In order for you to be able to understand and handle problems both in large and small scales, differences between analytical and large-scale problem inquiries will be illustrated. Guest lecturer with much insight into those specific techniques will be invited in order raise the quality of the course.
Intended learning outcomes
After passing the course, the student should be able to:
- Explain different protein separation methods, their properties, advantages and disadvantages and when it is most appropriate to use them.
- Explain the different analysis methods reviewed during the course, their properties, advantages and disadvantages
- Plan a working cleaning procedure for a protein with consideration to what has been learned in the course.
- Plan characterization of the purified protein and be able to take into consideration the available methods’ advantages and restrictions
- Carry out a lab which includes many of those reviewed techniques
- calculate gains/losses in a purification process and suggest improvements in order to reduce losses
Write a report which sheds light on the methods used and interpret data from the purification/analyses done
Literature and preparations
Specific prerequisites
Recommended prerequisites
BB1010 Introduction to Biotechnology
or
BB1050 Biotechnology and BB1080 Biochemistry, Theory
or
BB1090 Biochemistry, Theory
or
BB2050 Biochemistry
Equipment
Literature
Information about litterature will be given athttps://www.kth.se/social/course/BB1130/
at least three weeks prior to the start of the course
Examination and completion
If the course is discontinued, students may request to be examined during the following two academic years.
Grading scale
Examination
- LAB1 - Laboratory Work, 3.0 credits, grading scale: A, B, C, D, E, FX, F
- TEN1 - Examination, 4.0 credits, grading scale: A, B, C, D, E, FX, F
Based on recommendation from KTH’s coordinator for disabilities, the examiner will decide how to adapt an examination for students with documented disability.
The examiner may apply another examination format when re-examining individual students.
Other requirements for final grade
A written exam (TENA; 4 credits, grading scale A-F), Lab course (LABA; 3 credits, grading scale Pass/Fail).
Opportunity to complete the requirements via supplementary examination
Opportunity to raise an approved grade via renewed examination
Examiner
Ethical approach
- All members of a group are responsible for the group's work.
- In any assessment, every student shall honestly disclose any help received and sources used.
- In an oral assessment, every student shall be able to present and answer questions about the entire assignment and solution.
Further information
Course room in Canvas
Offered by
Main field of study
Education cycle
Add-on studies
Contact
Supplementary information
Students are required to sign up at least two weeks in advance for examination.
The course is given provided at least seven students are admitted.
Overlaps with BB1210-Purification of Biomolecules