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BB1130 Analysis and Purification of Biomolecules 7.0 credits

Analys och rening av biomolekyler

  • Educational level

    First cycle

  • Academic level (A-D)

    C

  • Subject area

    Biotechnology
    Techonology

  • Grade scale

    A, B, C, D, E, FX, F

Course offerings

Spring 13 for programme students

Spring 13 for programme students

  • Periods

    Spring 13 P3 (7.0 credits)

  • Application code

    61427

  • Start date

    2013 week: 2

  • End date

    2013 week: 11

  • Language of instruction

    English

  • Campus

    KTH Campus

  • Number of lectures

    30 (preliminary)

  • Number of exercises

  • Tutoring time

    Daytime

  • Form of study

    Normal

  • Number of places *

    7 - 60

    *) The Course date may be cancelled if number of admitted are less than minimum of places. If there are more applicants than number of places selection will be made.

  • Schedule

    Schedule (new window)

  • Course responsible

    Jenny Ottosson Takanen <jennyot@kth.se>

  • Teacher

    Jenny Ottosson Takanen <jennyot@kth.se>

  • Target group

    TMBIM study year 1, CBIOT (MBI), TIEMM study year 1 (BIIA)

Spring 14 for programme students

Learning outcomes

After passing the course, the student should be able to:

  • Explain different protein separation methods, their properties, advantages and disadvantages and when it is most appropriate to use them.
  • Explain the different analysis methods reviewed during the course, their properties, advantages and disadvantages
  • Plan a working cleaning procedure for a protein with consideration to what has been learned in the course.
  • Plan characterization of the purified protein and be able to take into consideration the available methods’ advantages and restrictions
  • Carry out a lab which includes many of those reviewed techniques
  • calculate gains/losses in a purification process and suggest improvements in order to reduce losses

 Write a report which sheds light on the methods used and interpret data from the purification/analyses done

Course main content

This course is focused on the methods with constitute the foundation for purification and analysis of biological macro molecules, such as nucleic acids and proteins.  The techniques which will be discussed often all exist within both biochemical/biotechnological research and industry.  During the course these methods will be described and discussed during the lectures, labs and a smaller project.  The course book will be complemented with descriptions from other literature and with relevant case studies from research.  If the opportunity arises, a visit to a company will be arranged.  In order for you to be able to understand and handle problems both in large and small scales, differences between analytical and large-scale problem inquiries will be illustrated.  Guest lecturer with much insight into those specific techniques will be invited in order raise the quality of the course.

Eligibility

Prerequisites

BB1010 Introduction to Biotechnology
or
BB1050 Biotechnology and BB1080 Biochemistry, Theory
or 
BB1090 Biochemistry, Theory 
or
BB2050 Biochemistry

Literature

Information about litterature will be given athttps://www.kth.se/social/course/BB1130/ 

at least three weeks prior to the start of the course

Examination

  • LAB1 - Laboratory Work, 3.0 credits, grade scale: A, B, C, D, E, FX, F
  • TEN1 - Examination, 4.0 credits, grade scale: A, B, C, D, E, FX, F

Requirements for final grade

A written exam (TENA; 4 credits, grading scale A-F), Lab course (LABA; 3 credits, grading scale Pass/Fail).

Offered by

BIO/Biotechnology

Contact

Jenny Ottosson Takanen, jennyot@kth.se

Examiner

Sophia Hober <sophia@kth.se>

Supplementary information

Students are required to sign up at least two weeks in advance for examination.

The course is given provided at least seven students are admitted.

Version

Course plan valid from: Spring 12.
Examination information valid from: Autumn 09.