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BB2040 Biochemical Analyses and Separation Techniques 7.5 credits

Biokemisk analys och separationsteknik

Please note

This course is in the process of being cancelled.

  • Educational level

    Second cycle

  • Academic level (A-D)

    D

  • Subject area

    Biotechnology

  • Grade scale

    A, B, C, D, E, FX, F

Last planned examination: Autumn 12

At present this course is not scheduled to be offered.

Learning outcomes

After passing the course, the student should be able to:

  • Explain different protein separation methods, their properties, advantages and disadvantages and when it is most appropriate to use them.
  • Explain the different analysis methods reviewed during the course, their properties, advantages and disadvantages
  • Plan a working cleaning procedure for a protein with consideration to what has been learned in the course.
  • Plan characterization of the purified protein and be able to take into consideration the available methods’ advantages and restrictions
  • Carry out a lab which includes many of those reviewed techniques
  • calculate gains/losses in a purification process and suggest improvements in order to reduce losses

 Write a report which sheds light on the methods used and interpret data from the purification/analyses done

Course main content

This course is focused on the methods with constitute the foundation for purification and analysis of biological macro molecules, such as nucleic acids and proteins.  The techniques which will be discussed often all exist within both biochemical/biotechnological research and industry.  During the course these methods will be described and discussed during the lectures, labs and a smaller project.  The course book will be complemented with descriptions from other literature and with relevant case studies from research.  If the opportunity arises, a visit to a company will be arranged.  In order for you to be able to understand and handle problems both in large and small scales, differences between analytical and large-scale problem inquiries will be illustrated.  Guest lecturer with much insight into those specific techniques will be invited in order raise the quality of the course.

Eligibility

Prerequisites

BB1010 Introduction to Biotechnology or Biotechnology BB1050 and BB1080 Biochemistry, Theory or BB1090 Biochemistry, Theory or BB2050 Biochemistry

Literature

Practical Biochemistry, 6th ed.(2005), Wilson and Walker

Examination

  • LABA - Laboratory Work, 3.0 credits, grade scale: A, B, C, D, E, FX, F
  • TENA - Examination, 4.5 credits, grade scale: A, B, C, D, E, FX, F

Requirements for final grade

A written exam (TENA; 4,5 credits, grading scale A-F), Lab course (LABA; 3 credits, grading scale Pass/Fail).

Offered by

BIO/Biotechnology

Contact

Torbjörn Gräslund, torbjorn.graslund@biotech.kth.se, tel. 553 783 32

Examiner

Sophia Hober, sophia.hober@biotech.kth.se

Version

Course plan valid from: Autumn 07.
Examination information valid from: Autumn 07.