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Publications

All publications from the Competence Centre for Advanced BioProduction by Continuous Processing.

Peer reviewed publications of AdBIOPRO in journals and proceedings

  1. Brechmann, N. A.; Eriksson, P.-O.; Eriksson, K.; Oscarsson, S.; Buijs, J.; Shokri, A.; Hjälm, G.; Chotteau, V. 2019. Pilot-scale process for magnetic bead purification of antibodies directly from non-clarified CHO cell culture. Biotechnology Progress 2019, 35, (3), e2775
  2. Brechmann, N.A., Schwarz, H., Eriksson, P.-O., Eriksson, K., Shokri, A., Chotteau, V. 2021. Antibody capture process based on magnetic beads from very high cell density suspension. Biotechnology and Bioengineering. https://doi.org/10.1002/bit.27776
  3. Brechmann, N.A., Jansson, M., Hägg, A., Hicks, R., Hyllner, J., Eriksson, K. and Chotteau, V., 2022. Proof-of-Concept of a Novel Cell Separation Technology Using Magnetic Agarose-Based Beads. Magnetochemistry, 8(3), p.34.
  4. Colin K, Hjalmarsson H, Chotteau V. Gaussian process modelling of macroscopic kinetics: a better-tailored kernel for Monod-type kinetics. submitted to MATHMOD/ IFAC 2022
  5. Eisenhut, P., Mebrahtu, A., Moradi Barzadd, M., Thalén, N., Klanert, G., Weinguny, M., Sandegren, A., Su, C., Hatton, D., Borth, N. and Rockberg, J., 2020. Systematic use of synthetic 5′-UTR RNA structures to tune protein translation improves yield and quality of complex proteins in mammalian cell factories. Nucleic acids research, 48(20), pp.e119-e119.
  6. Gomis-Fons, J., Löfgren, A., Andersson, N., Nilsson, B., Berghard, L., Wood, S. 2019. Integration of a complete downstream process for the automated lab-scale production of a recombinant protein. J Biotech 301 pp 45-51
  7. Gomis-Fons, J., Schwarz, H., Zhang, L., Andersson, N., Nilsson, B., Castan, A., Solbrand, A., Stevenson, J., Chotteau, V. 2020. Model-based design and control of a small-scale integrated continuous end-to-end mAb platform. Biotechnology Progress
  8. Gomis-Fons, J., Andersson, N., Nilsson, B., 2020. Optimization study on periodic counter-current chromatography integrated in a monoclonal antibody downstream process. Journal of Chromatography A, 1621
  9. Gomis-Fons, J., Yamanee-Nolin, M., Andersson, N. and Nilsson, B. Optimal loading flow rate trajectory in monoclonal antibody capture chromatography. Journal of Chromatography A. 2021. 1635, p.461760
  10. Hagrot, E., Oddsdóttir, H. Æ., Mäkinen, M., Forsgren, A. and Chotteau, V. 2019. Novel column generation-based optimization approach for poly-pathway kinetic model applied to CHO cell culture. Metabolic engineering communications, 8:e00083
  11. Ladd, B., Bowes, K., Lundgren, M., Gräslund, T., Chotteau, V., 2022. Proof-of-concept of continuous transfection for adeno-associated virus production in microcarrier-based culture, Processes 2022, 10(3), 515; doi.org/10.3390/pr10030515
  12. Löfgren, A., Gomis-Fons, J., Andersson, N., Nilsson, B., Berghard, L. and Hägglund, C.L. 2020. An integrated continuous downstream process with real‐time control: A case study with periodic countercurrent chromatography and continuous virus inactivation. Biotechnology and Bioengineering. https://doi.org/10.1002/bit.27681
  13. Malm, M., Saghaleyni, R., Lundqvist, M., Giudici, M., Chotteau, V., Field, R., Varley, P.G., Hatton, D., Grassi, L., Svensson, T., Nielsen, J. and Rockberg J, 2020. Evolution from adherent to suspension: systems biology of HEK293 cell line development. Scientific reports, 10(1), pp.1-15.
  14. Moradi, M., Lundqvist, M., Harris, C., Malm, M., Volk, A.L., Thalén, N., Chotteau, V., Grassi, L., Smith, A., Abadi, M.L. and Lambiase, G., Gibson, S., Hatton, D., Rockberg, J. 2022. Autophagy and intracellular product degradation genes identified by systems biology analysis reduce aggregation of bispecific antibody in CHO cells, 2022, New Biotechnol., in press, doi.org/10.1016/j.nbt.2022.01.010.
  15. Malm, M., Kuo, CC, Barzadd, MM, Mebrahtu, A, Wistabacka, N, Razavi, R, Volk, A-L, Lundqvist, M, Kotol, D, Edfors, F, Gräslund, T, Chotteau, V, ..., Rockberg. Harnessing secretory pathway differences between HEK293 and CHO to rescue production of difficult to express proteins, Harnessing secretory pathway differences between HEK293 and CHO to rescue production of difficult to express proteins. Metabolic Engineering, 2022 accepted
  16. Moreno-González, M., Keulen, D., Gomis-Fons, J., Gomez, G.L., Nilsson, B. and Ottens, M. 2021. Continuous adsorption in food industry: The recovery of sinapic acid from rapeseed meal extract. Separation and Purification Technology. 254, p.117403.
  17. Nahi, H., Chrobok, M., Meinke, S., Gran, C., Marquardt, N., Afram, G., Sutlu, T., Gilljam, M., Stellan, B., Wagner, A., K., Blomberg, P., Holmqvist, P.-H., Walther-Jallow, L., Mellström, K., Liwing, J., Gustafsson, C., Månsson, R., Klimkowska, M., Gahrton, G., Lund, J., Ljungman, P., Ljunggren, H.-G., Alici, E. 2022. Autologous NK cells as consolidation therapy following stem cell transplantation in multiple myeloma. Cell Reports Medicine Volume 3, Issue 2, 10050.
  18. Pinto, I.F., Mikkonen, S., Josefsson, L., Mäkinen, M.E., Soares, R.R., Russom, A., Emmer, Å. and Chotteau, V., 2021. Knowing more from less: miniaturization of ligand-binding assays and electrophoresis as new paradigms for at-line monitoring and control of mammalian cell bioprocesses. Current Opinion in Biotechnology, 71, pp.55-64.
  19. Rodrigues D. and Hjalmarsson H. Stability and performance analysis of control based on incomplete models. Proc. 12th IFAC Symposium on Dynamics and Control of Process Systems (DYCOPS), Florianopolis, Brazil, April 2019
  20. Rodrigues, D., Abdalmoaty, M.R. and Hjalmarsson, H.. Toward tractable global solutions to maximum-likelihood estimation problems via sparse sum-of-squares relaxations. Proc. 58th IEEE Conference on Decision and Control (CDC), Nice, France, Dec 2019
  21. Rodrigues, D., Abdalmoaty, M.R. and Hjalmarsson, H.. Toward Tractable Global Solutions to Bayesian Point Estimation Problems via Sparse Sum-of-Squares Relaxations . Proc. 2020 American Control Conference (ACC), Denver, CO, USA, July 2020. DOI: 10.23919/ACC45564.2020.9147484
  22. Rodrigues, D., Abdalmoaty, M.R., Jacobsen, E.W., Chotteau, V. and Hjalmarsson, H. 2021. An Integrated Approach for Modeling and Identification of Perfusion Bioreactors via Basis Flux Modes. Computers & Chemical Engineering. Jan 26:107238.
  23. Saghaleyni, R., Malm, M., Zrimec, J., Razavi, R., Wistbacka, N., Chotteau, V., ... & Rockberg, J. (2020). Enhanced metabolism and negative regulation of ER stress support higher erythropoietin production in HEK293 cells. Cell Reports 2022 accepted
  24. Särnlund, S., Jiang, Y. and Chotteau, V., 2021. Process intensification to produce a difficult‐to‐express therapeutic enzyme by high cell density perfusion or enhanced fed‐batch. Biotechnology and Bioengineering. https://doi.org/10.1002/bit.27806
  25. Scheffel, J., Kanje, S., Borin, J. and Hober, S. 2019. Optimization of a calcium-dependent Protein A-derived domain for mild antibody purification. MAbs, 11:1492-1501. DOI: 10.1080/19420862.2019.1662690
  26. Scheffel, J., and Hober, S. 2021. Highly selective Protein A resin allows for mild sodium chloride-mediated elution of antibodies. Journal of Chromatography A. 1637, p 461843
  27. Scheffel, J., Isaksson, M., Gomis-Fons, J., Schwarz, H., Andersson, N., Norén, B., Solbrand, A., Chotteau, V., Hober, S. and Nilsson, B., 2022. Design of an integrated continuous downstream process for acid-sensitive monoclonal antibodies based on a calcium-dependent Protein A ligand. Journal of Chromatography A, p.462806.
  28. Schwarz H, Gomis-Fons J, Isaksson M, Scheffel J, Andersson N, Andersson A, Castan A, Solbrand A, Hober S, Nilsson B, Chotteau V. Integrated continuous biomanufacturing on pilot scale for acid-sensitive monoclonal antibodies, under revision in Biotechnology and Bioengineering
  29. Schwarz H, Mäkinen M, Castan A, Chotteau V. Monitoring of Amino Acids and Antibody N-Glycosylation in High Cell Density Perfusion Culture based on Raman Spectroscopy, under revision in Biochemical Engineering Journal
  30. Volk, A.L., Mebrahtu, A., Ko, B.K., Lundqvist, M., Karlander, M., Lee, H.J., Frejd, F.Y., Kim, K.T., Lee, J.S. and Rockberg, J., 2021. Bispecific Antibody Molecule Inhibits Tumor Cell Proliferation More Efficiently Than the Two-Molecule Combination. Drugs in R&D, 21(2), pp.157-168.
  31. Wang, M., Jacobsen, E.W., Chotteau, V., and Hjalmarsson. H., A multi-step least-squares method for nonlinear rational models. Proc. American Control Conference (ACC), Philadelphia, PA, July 2019
  32. Wang, M., Jacobsen, E.W., Chotteau, V. and Hjalmarsson, H.. Unscented Bayes Methods for Hierarchical Gaussian Processes . Australian and New Zealand Control Conference (ANZCC) (pp. 137-142). IEEE. 2020. Gold Coast, QLD, Australia, Nov, 2020. DOI: 10.1109/ANZCC50923.2020.9318341
  33. Wang, M., Jacobsen, E., Chotteau, V., Hjalmarsson, H. . Estimation of Heteroscedastic Multilinear Systems. 59th IEEE Conference on Decision and Control (CDC). 2020. Jeju, Korea (South), Dec. 2020. DOI: 10.1109/CDC42340.2020.9304127
  34. Wang, M., Risuleo, R., S., Jacobsen, E., W., Chotteau, V., and Håkan Hjalmarsson. 2020. Identification of nonlinear kinetics of macroscopic bio-reactions using multilinear Gaussian processes. Computers & Chemical Engineering 133: 106671
  35. Wang, Y. and Jacobsen E.W., A Geometric Approach to Target Identification, 8th IFAC Conference on Foundations of Systems Biology, Valencia, Spain, Oct, 2019
  36. Wang, Y., Pasquini, M., Chotteau, V., Hjalmarsson, H., Jacobsen, E.. Robust optimization with optimal experiment design - with application to continuous biopharmaceutical production", accepted at DYCOPS 2022
  37. Zhan, C., Bidkhori, G., Schwarz, H., Malm, M., Mebrahtu, A., Field, R., Rockberg, J., Chotteau, V. 2020. Low shear stress increases recombinant protein production and high shear stress increases apoptosis in human cells. iScience. 101653 DOI:https://doi.org/10.1016/j.isci.2020.101653
  38. Zhang, L , Castan , A, Stevenson , J, Chatzissavidou , N, Vilaplana , F, Chotteau , V, Combined Effects of Glycosylation Precursors and Lactate on the Glycoprofile of IgG Produced by CHO Cells. 2019. Journal of Biotechnology 289, 71-79
  39. Zhang, L., Wang, M., Castan, A., Stevenson, J., Chatzissavidou, N., Hjalmarsson, H., Vilaplana, F., and Chotteau, V. 2020. Glycan Residues Balance Analysis-GReBA: A novel model for the N-linked glycosylation of IgG produced by CHO cells. Metabolic engineering 57: 118-128
  40. Zhang, L., Schwarz, H., Wang, M., Castan, A., Hjalmarsson, H. and Chotteau, V. Control of IgG glycosylation in CHO cell perfusion cultures by GReBA mathematical model supported by a novel targeted feed, TAFE . Metabolic Engineering. 2020. https://doi.org/10.1016/j.ymben.2020.11.004
  41. Zhang, L, Wang ML, Castan A, Hjalmarsson H, Chotteau V. 2021. Probabilistic Model by Bayesian Network for the Prediction of Antibody Glycosylation in Perfusion and Fed-Batch Cell Cultures, Biotechnology and Bioengineering. https://doi.org/10.1002/bit.27769

Book chapters

Chotteau, V., Hagrot, E., Zhang, L. and Mäkinen, M.E., 2021. Mathematical Modelling of Cell Culture Processes. In Cell Culture Engineering and Technology (pp. 431-466). Springer, Cham.

Presentations at international and national conferences

  1. Bernt Nilsson. General platform for development of integrated downstream processes. Lecture at ACS, New Orleans, USA, March 2018
  2. Bernt Nilsson. Integrated downstream processes of biologics. Lecture at Biologics World Nordic, Copenhagen, Denmark, Feb 2018
  3. Bernt Nilsson. Small scale platform for development of integrated DSP. Lecture at ESBES, Lissabon, Portugal, Sept 2018
  4. Bernt Nilsson. Smart and integrated downstream processing of new biologics. Lecture at NGPP, Aachen, Germany, July 2018
  5. Mingliang Wang. A Multi-linear Gaussian Process for Model Selection of Nonlinear Kinetics in Bio-reactions. Poster at European Research Network on System Identification ERNSI, Cambridge, U.K., Sept, 2018
  6. Sophia Hober. A novel IgG binding purification matrix for mild elution. Lecture at 17th PepTalk-The Protein Science Week, SanDiego, Jan 2018
  7. Sophia Hober. A Protein A-Derived Domain with Calcium-Dependent Affinity for Use in Antibody Purification. Lecture at Bioprocessing Summit Europe Lisbon. March 2018
  8. Veronique Chotteau. High cell density perfusion process of HEK 293 cells. Lecture at Bioprocess International, KNect365, September 4-7, 2018
  9. Veronique Chotteau. Real time monitoring of cell culture – an overview. Lecture at ACTIP, Stockholm, Sweden, December 13-14, 2018
  10. Julia Scheffel. Optimization of an IgG-binding protein domain with calcium-dependent behavior for antibody purification. Poster at Bioprocessing Summit Europe Lisbon. March 2018
  11. Johan Rockberg. CHO cell secretion - product and exo proteome monitoring. Lecture at Bioengineering Platforms, Brisbane April 2018
  12. Johan Rockberg. Production of biopharmaceuticals in an intensified perfusion process of HEK 293 cells. Lecture at Cell culture Engineering XIV, May 2018
  13. Veronique Chotteau. Very high cell density perfusion culture for the production of biologics using CHO cells or HEK293 cells. Lecture at 15th Annual Optimizing Cell Culture Technology – Bioprocess Summit, Aug 12-13, 2019, Boston, MA
  14. Bernt Nilsson. Integrated continuous downstream process development. Lecture at Biologics World Nordic, Stockholm, March 2019
  15. Bernt Nilsson. Small scale integrated downstream process with online realtime release. Lecture at ACS, Orlando, FL, USA, April 2019
  16. Brian Ladd. Adeno-associated virus production in stirred tank bioreactor. Lecture at AdBIOPRO Workshop, Stockholm, Sept 2019
  17. Daniel Smith. Plasmid DNA and Viral Vector Manufacture. Lecture at University College London, 2019
  18. Darrell Sleep. Challenges of viral vector manufacturing - a CDMO perspective. Lecture at Cell and gene therapy symposia: Meeting the challenges of scale. London UK, Nov 2019
  19. Diogo Rodrigues. Stability and Performance Analysis of Control Based on Incomplete Models. Lecture at Proc. 12th IFAC Symposium on Dynamics and Control of Process Systems (DYCOPS), Florianópolis, Brazil, April, 2019
  20. Diogo Rodrigues. Toward Tractable Global Solutions to Maximum-Likelihood Estimation Problems via Sparse Sum-of-Squares Relaxations. Lecture at Proc. 58th IEEE Conference on Decision and Control (CDC), Nice France, Dec, 2019
  21. Hubert Schwarz. A small-scale mAb platform with a continuous and integrated design. Poster at 26th ESACT, Copenhagen, Denmark, May 2019
  22. Hubert Schwarz. High cell density perfusion of human/animal cells in small-scale bioreactors. Poster at 26th ESACT, Copenhagen, Denmark, May 2019
  23. Hubert Schwarz. Amino acid feeding strategy in perfusion cultures to enhance monoclonal antibody production. Poster at Integrated Continuous Biomanufacturing, Brewster, MA, USA, Oct 2019
  24. Hubert Schwarz. Applications of small-scale bioreactors for high cell density perfusion process development. Lecture at ACS, Orlando, FL, USA, April 2019
  25. Hubert Schwarz. Strategy for targeted delivery of key nutrients in high cell density perfusion. Lecture at AdBIOPRO Workshop, Stockholm, Sept 2019
  26. Joaquin Gomis Fons. Optimization and Control of Continuous Downstream Processing for Antibody Purification. Lecture at AdBIOPRO Workshop, Stockholm, Sept 2019
  27. Joaquin Gomis Fons. Optimization study on periodic counter-current chromatography (PCC) integrated in a mAb downstream process. Short-talk&poster at Integrated Continuous Biomanufacturing, Brewster, MA, USA, Oct 2019
  28. Joaquin Gomis Fons. Small Scale End-To-End Mab Platform With A Continuous, Integrated And Compact Process. Poster at ACS, Orlando, FL, USA, April 2019
  29. Joaquin Gomis Fons. Small Scale End-To-End Mab Platform With A Continuous, Integrated And Compact Process. Poster at Bioprocessing Summit Europe, Lisbon, Portugal, March, 2019
  30. Julia Scheffel. Optimization of a calcium-dependent affinity ligand and its use in continuous capture. Lecture at AdBIOPRO Workshop, Stockholm, Sept 2019
  31. Julia Scheffel. Optimization of a calcium-dependent Protein A-derived domain for mild antibody purification. Poster at Affinity, Stockholm, June 2019
  32. Julia Scheffel. Optimization of a calcium-dependent Protein A-derived domain for mild antibody purification. Poster at Bioprocess Summit, Boston, MA, USA, Aug 2019
  33. Liang Zhang. Combined effects of glycosylation precursors and lactate on the glycoprofile of IgG produced by CHO cells. Poster at 26th ESACT, Copenhagen, Denmark, May 2019
  34. Liang Zhang. Glycan residues balance analysis: A novel model for the N-linked glycosylation of IgG produced by CHO cells. Poster at 26th ESACT, Copenhagen, Denmark, May 2019
  35. Liang Zhang. Glycan Residues Balance Analysis: A Novel Model for the N-linked Glycosylation of IgG Produced by CHO Cells. Lecture at AdBIOPRO Workshop, Stockholm, Sept 2019
  36. Mingliang Wang. Experimental design and estimation of nonlinear kinetics in process of mammalian cell culture. Lecture at AdBIOPRO Workshop, Stockholm, Sept 2019
  37. Mingliang Wang. A Multi-Step Least-Squares Method for Nonlinear Rational Models. Lecture at 2019 Annual American Control Conference (ACC), Philadelphia, PA, USA, July, 2019
  38. Nils Brechmann. Continuous antibody capture step based on magnetic beads. Poster at Integrated Continuous Biomanufacturing, Brewster, MA, USA, Oct 2019
  39. Nils Brechmann. Continuous antibody capture step based on magnetic beads. Lecture at AdBIOPRO Workshop, Stockholm, Sept 2019
  40. Nils Brechmann. Magnetic bead purification of mAb from CHO cell broth at pilot scale . Lecture at 26th ESACT, Copenhagen, Denmark, May 2019
  41. Per-Henrik Holmqvist. Towards Large-scale Manufacturing of Cell Therapy Products. Lecture at AdBIOPRO Workshop, Stockholm, Sept 2019
  42. Pontus Blomberg. Manufacturing of Advanced Therapy Medicinal Products (ATMPs) in a European Academic Setting. Lecture at 4th Annual Cell and Gene Therapy Symposium, Vellore, 2019
  43. Sigrid Lundin. A CHO high cell density perfusion process with improved glycan profile. Poster at 26th ESACT, Copenhagen, Denmark, May 2019
  44. Sigrid Lundin. A high-cell density perfusion process for production of a difficult-to-express enzyme . Lecture at AdBIOPRO Workshop, Stockholm, Sept 2019
  45. Sophia Hober. Optimization and utilization of an IgG-binding, Protein A-based purification matrix. Lecture at Biologics World Nordic, Stockholm, March 2019
  46. Sophia Hober. The development of small protein domains for biotechnological and medical use. Lecture at ISPPP18, Berlin, November 2019
  47. Veronique Chotteau. Strategy for targeted delivery of key nutrients in high cell density perfusion. Lecture at Integrated Continuous Biomanufacturing, Brewster, MA, USA, Oct 2019
  48. Veronique Chotteau. Dynamical mathematical model of CHO cell culture. Lecture at Bioprocess International BPI, Boston, MA, USA, Sept 2019
  49. Veronique Chotteau. High cell density culture for the production of biologics. Lecture at Mammalian Cell Bioprocessing MBI, UCL, London, UK, Jan 2019
  50. Veronique Chotteau. High cell density perfusion culture and integrated system for the production of biologics. Lecture at Biologics World Nordic, Stockholm, March 2019
  51. Veronique Chotteau. Integrated small-scale end-to-end system for mAb production in process development. Lecture at Bioprocess International BPI West, Santa Clara, CA, USA, March 2019
  52. Veronique Chotteau. Very High Cell Density Perfusion Culture for the Production of Biologics Using CHO Cells or HEK293 Cells. Lecture at New Horizons in Biologics and Bioprocessing, Stockholm, Dec, 2019
  53. Yu Wang. A Geometric Approach to Target Identification. Lecture at 8th IFAC Conference on Foundations of Systems Biology, Valencia, Spain, Oct, 2019
  54. Johan Rockberg. Droplet and solid phase based-methods for selection and production of next generation biologics. Poster at Affinity, Stockholm, June 2019
  55. Johan Rockberg. HEK293 cell lines allow rescue of proteins that are difficult to produce in CHO. Poster at ESACT 2019, Copenhagen
  56. Veronique Chotteau. Omics for high cell density and shear stress in perfusion processes. lecture at ESACT 2019, Copenhagen
  57. Veronique Chotteau. Development of perfusion and integrated USP-DSP processes in high cell density small-scale bioreactors. lecture at Continuously improving bioprocesses – Pall Biotech, March 21, 2019, Stockholm, Sweden
  58. Veronique Chotteau. Very High Cell Density Perfusion Culture for the Production of Biologics Using CHO Cells or HEK293 Cells. Lecture at Bioprocess Summit, Boston, MA, USA, Aug 2019
  59. Johan Rockberg. SAMURAI (Solid-phase Assisted Mutagenesis by Uracil Restriction for Accurate Integration) - A Method for Generation of High-Quality Antibody Libraries. Lecture at PEGS Europe, Lisbon, Nov 2019
  60. Nils Brechmann. Purification process including cell separation using protein A magnetic beads. Lecture at ACS-BIOT San Franscisco, CA, USA, Aug 2020, web
  61. Veronique Chotteau. Raman Spectroscopy for Online Monitoring of Cell Culture Perfusion Processes. Lecture at Second Biomanufacturing Forum, Endress+Hauser, Oct 20-21, 2020, webinar (instead of Switzerland)
  62. Diogo Rodrigues . Toward Tractable Global Solutions to Bayesian Point Estimation Problems via Sparse Sum-of-Squares Relaxations . Lecture at Proc. 2020 American Control Conference (ACC)
  63. Mingliang Wang . Unscented Bayes Methods for Hierarchical Gaussian Processes . Lecture at Australian and New Zeeland Control Conference
  64. Veronique Chotteau. Developing and understanding perfusion processes. Lecture at BPI Digital week webinar, USA, Dec 2020
  65. Veronique Chotteau. Paradigm shift towards continuous process bioproduction. Lecture at Transformation of pharmaceutical industry for climat and economic competition, Sept 2020, web
  66. Yu Wang. Inferring individual network edges - with application to target identification in gene networks. Lecture at 21st IFAC World Congress, Berlin, Jul 11-17, 2020
  67. Sophia Hober. Optimization and utilization of an IgG-binding, Protein A-based purification matrix . Lecture at Bioprocessing Summit Europe, digital, July 2020
  68. Sophia Hober. Optimization and utilization of an IgG-binding, Protein A-based purification matrix . Lecture at PEGS Europe 2020, digital, Nov 2020
  69. Mingliang Wang. Estimation of Heteroscedastic Multilinear Systems. Lecture at 59th IEEE Conference on Decision and Control (CDC)
  70. Julia Scheffel. A calcium- dependent Protein A-derived ligand for mild purification of antibodies and antibody fragments. Lecture at Affinity 2021, Virtual conference
  71. Julia Scheffel. A calcium- dependent Protein A-derived ligand for mild purification of antibodies. Lecture at ISPPP 2021, Porto
  72. Julia Scheffel. Calcium-dependent affinity ligands for the mild purification of antibody fragments. Lecture at AdBIOPRO Workshop 2021 Bioproduction of Therapeutic Biologics, Stockholm
  73. Veronique Chotteau. Continuous integrated upstream-downstream process gives high productivity and small footprint for antibody production. Lecture at New Horizons in Biologics & Bioprocessing 2021, Stockholm, Dec 2021
  74. Veronique Chotteau. Continuous high cell density perfusion culture: Potential for bioproduction and overview of process development. Lecture at Integrated Continuous Biomanufacturing webinar, June 2021
  75. Veronique Chotteau. On-line monitoring of metabolites and glycosylation in high cell density perfusion process. Lecture at BioProcess International (BPI) Boston event, Boston, MA, USA, Sept. 2021
  76. Veronique Chotteau. Mathematical Modelling of a CHO Cell Perfusion Process. Lecture at Bioprocessing Summit, Boston, MA, USA, August, 2021
  77. Bernt Nilsson. Integrated Continous Biomanufacturing of antibody - pilot study at Testa Centre. Lecture at Vinnova
  78. Hubert Schwarz. Developing high cell density perfusion process for the production of monoclonal antibody within our Competence centre AdBIOPRO. Lecture at BCME Biologics Contract Manufacturing Europe, IMAPAC, Dec 2021
  79. Nils Brechmann. Antibody capture process based on magnetic beads from culture at very high cell density > 100 x 1E6 cells/mL suspension. Lecture at ACS, Atlanta, August 2021
  80. Magnus Lundqvist. Harnessing transcriptomics to improve production of biologics. Lecture at AdBIOPRO Workshop 2021 Bioproduction of Therapeutic Biologics, Stockholm
  81. Kévin Colin. Metabolic Flux Analysis modelling of CHO cell process in pseudo-perfusion mode: a novel approach based on transcriptomics. Lecture at AdBIOPRO Workshop 2021 Bioproduction of Therapeutic Biologics, Stockholm
  82. Mirko Pasquini & Yu Wang. Towards a model-based approach to optimize the medium composition in continuous bioprocess. Lecture at AdBIOPRO Workshop 2021 Bioproduction of Therapeutic Biologics, Stockholm
  83. Andreas Castan. Using perfusion for process intensification strategies across the entire upstream workflow. Lecture at AdBIOPRO Workshop 2021 Bioproduction of Therapeutic Biologics, Stockholm
  84. Sigrid Särnlund. Process intensification to produce a difficult-to-express therapeutic enzyme by high cell density perfusion or enhanced fed-batch. Lecture at AdBIOPRO Workshop 2021 Bioproduction of Therapeutic Biologics, Stockholm
  85. Joaquin Gomis Fons, Madelène Isaksson, Julia Scheffel, Hubert Schwarz. Mild integrated continuous biomanufacturing of monoclonal antibodies at pilot scale. Lecture at AdBIOPRO Workshop 2021 Bioproduction of Therapeutic Biologics, Stockholm
  86. Nils Brechmann. Antibody capture process based on magnetic beads from suspension cultures at densities > 100 x 1E6 cells/mL. Lecture at AdBIOPRO Workshop 2021 Bioproduction of Therapeutic Biologics, Stockholm
  87. Per-Henrik Holmqvist. Developing a robust scale-up and scale-out method for manufacturing of cell therapy products. Lecture at AdBIOPRO Workshop 2021 Bioproduction of Therapeutic Biologics, Stockholm
  88. Brian Ladd. Continuous manufacturing of recombinant adeno-associated viral vectors. Lecture at AdBIOPRO Workshop 2021 Bioproduction of Therapeutic Biologics, Stockholm
  89. Johan Rockberg. Producing Difficult, Active And Non-Aggregating Proteins –Lessons From Omics Of Cho And Hek293. Lecture at PEACE 2021, Qubec Canada, Digital
  90. Johan Rockberg. A Platform for Precise Tuning of Protein Translation in Mammalian Cells for Improved Product Quality and Yield. Lecture at PEGS.Europe 2021, Barcenlona, Spain
  91. Johan Rockberg. Producing Proteins Difficult To Express – How Omics Can Help You. Lecture at EFB 2021, digitally
  92. Sophia Hober. A Novel Protein A-based Purification Matrix for Mild Purification of Antibodies
     applied in an integrated continuous biomanufacturing setup. Lecture at 20th PepTalk-The Protein Science Week, San Diego, Jan 2022
  93. Sophia Hober. A Novel Protein A-Based Purification Matrix for Mild Purification of Biologicals – From a Novel Affinity Molecule to Continuous Pilot Scale Purification. Lecture at Bioprocessing Summit Europe, Barcelona, March 2022
  94. Veronique Chotteau. Integrated upstream-downstream process, the journey from lab scale to pilot scale. Lecture at Bioprocessing Summit Europe, Barcelona, March 2022
  95. Veronique Chotteau. Smart tools for high cell density perfusion process producing monoclonal antibodies. Plenary lecture at BioProScale symposium, Berlin, March 2022
  96. Nils Brechmann. Proof-of-concept of a novel scalable magnetic bead-based cell separation technology . Poster at ECI Advancing manufacture of cell and gene therapies VII, Coronado, CA, USA, February 2022
  97. Brian Ladd. An efficient microcarrier based Adeno-associated virus production method. Poster at ECI Advancing manufacture of cell and gene therapies VII, Coronado, CA, USA, February 2022
  98. Kristofer Eriksson. Next-Generation Integrated Antibody Capture Process Based on Magnetic Beads from High Cell Density Cultures. Lecture at 20th PepTalk-The Protein Science Week, San Diego, Jan 2022
  99. Yu Wang. Robust optimization with optimal experiment design - with application to continuous biopharmaceutical production. Lecture at 13th IFAC Symposium on Dynamics and Control of Process Systems, including Biosystems, (DYCOPS 2022), accepted
  100. Kevin Colin. Gaussian process modeling of macroscopic kinetics: a better-tailored kernel for Monod-type kinetics. Lecture at 10th Vienna International Conference on Mathematical Modelling (MATHMOD 2022), July 2022
  101. Veronique Chotteau. Development approach of an intensified integrated continuous process for pilot scale. Lecture at BioProcess International Europe, Vienna, May 2022
  102. Hubert Schwarz. Raman-based monitoring of amino acids and antibody N-glycosylation in high cell density perfusion culture. Lecture at BioProcess International Europe, Vienna, May 2022

PhD Thesis

  1. Joaquin Gomis Fons. 2022. Design and Control of Integrated Continuous Processes for the Purification of Biopharmaceuticals. (Doctoral dissertation). Lund, Sweden: Lund University
  2. Erika Hagrot. 2019. Macroscopic models of Chinese hamster ovary cell cultures. (Doctoral dissertation). Stockholm, Sweden: KTH Royal Institute of Technology
  3. Liang Zhang. 2020. Development of mathematical modelling for the glycosylation of IgG in CHO cell cultures. (Doctoral dissertation). Stockholm, Sweden: KTH Royal Institute of Technology