Cell-Ring - Close proximity photonic sensing of single-cell secretion

Biological processes are dynamic and involve the interactions of cells with each other and their environment. To study the dynamics of cell signaling, measurements at the single cell level are necessary, since intra population variability and diffusion limitations tend to smear out the aggregate biochemical response of large cell populations. The aim of the Cell-Ring project is to create a nano-photonic sensor to study the biomolecule secretion of single cells in real-time.

Duration: 2012-2017

 

The sensor works by trapping light in a ring that encircles the cell at close range. When secreted biomolecules bind to the ring's surface, the speed of the light captured is altered.
The sensor works by trapping light in a ring that encircles the cell at close range. When secreted biomolecules bind to the ring's surface, the speed of the light captured is altered.

Label-free biosensors are uniquely suited for monitoring biomolecular dynamics in real-time. However, current sensors are not able to resolve the minute amount of protein secreted from single cells. By virtue of their cell-like dimensions, nano-photonic sensors are able to sample the secreted biomaterial close to the cell. In combination with their low protein mass detection limit, their small size gives these sensors a unique potential for resolving the signaling dynamics.

The aim of the Cell-Ring project is to develop a Lab-on-a-Chip, in which nano-photonic sensors are integrated with a microfluidic cell and liquid handling system. By novel design and fabrication approaches, we aim to improve the detection limit of the photonic sensors, to be able to measure even proteins secreted at low levels with a useful time resolution. Such a tool enables the direct study of the cell´s biochemical signaling in response to stimuli, and is thus of great interest for fundamental research in biology and for drug development, in particular. 

In order to fabricate the complete Lab-on-a-Chip system, we develop a novel method for the integration of polymer microfluidics and cell handling with silicon photonic sensors, compatible with wafer scale production methods. The microfluidics layer was fabricated in a single step in the recently introduced  OSTE polymer  and dry-bonded to silicon photonic sensors. This integration method can match the microfluidics to the size scale of current photonic silicon sensors.

The fabrication process for the integrated chip developed in this project. A microfluidic layer is fabricated in a single step process in OSTE and dry-bonded to a silicon photonic sensor chip.

Project sponsor

Swedish Research Council (Vetenskapsrådet)

Project partner

KTH Cell Physics

Project members

Carlos Errando-Herranz

Kristinn B. Gylfason

Tommy Haraldsson

Hans Sohlström

Publications related to this project