F6L5013 Structural Biotechnology - Electron Microscopy 7.5 credits

Strukturell bioteknik - Elektronmikroskopi

Offering and execution

Course offering missing for current semester as well as for previous and coming semesters

Course information

Content and learning outcomes

Course contents *

The course is intended to give basic knowledge in the field of TEM as a platform for high-resolution applications. Lectures and seminars will give the necessary theoretical background while practical sessions introduce specimen preparation, instrument usage, and data processing.

Intended learning outcomes *

To introduce high resolution transmission electron microscopy (TEM).

Course Disposition

  • Image formation in TEM
  • Electron diffraction
  • Resolution
  • Electron optics and instrument design
  • Beam damage
  • Image acquisition
  • Specimen preparation
  • Basic crystallography
  • Image processing (image reconstruction, noise reduction, 3DEM, multivariate statistics)

Literature and preparations

Specific prerequisites *

Accepted for doctoral studies at KTH or Karolinska Institutet.

Recommended prerequisites

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Williams, D B & Carter, C B, Transmission Electron Microscopy, Plenum Press 1996, ISBN 0-306-45247-2.
Dorset, Douglas L, Structural Electron Crystallography, Plenum Press 1995, ISBN 0-306-45049-6.
Frank, Joachim, Three-Dimensional Electron Microscopy of Macromolecular Assemblies, Academic Press 1996, ISBN 0-12-265040-9.

Examination and completion

If the course is discontinued, students may request to be examined during the following two academic years.

Grading scale *

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Examination *

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Based on recommendation from KTH’s coordinator for disabilities, the examiner will decide how to adapt an examination for students with documented disability.

The examiner may apply another examination format when re-examining individual students.

Written examination.

Other requirements for final grade *

Passed practical exercise and certain percentage of correct answers on written exam.

Opportunity to complete the requirements via supplementary examination

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Opportunity to raise an approved grade via renewed examination

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Hans Hebert

Ethical approach *

  • All members of a group are responsible for the group's work.
  • In any assessment, every student shall honestly disclose any help received and sources used.
  • In an oral assessment, every student shall be able to present and answer questions about the entire assignment and solution.

Further information

Course web

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Offered by

STH/Structural Biotechnology

Main field of study *

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Education cycle *

Third cycle

Add-on studies

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Docent Philip Köck

Postgraduate course

Postgraduate courses at STH/Structural Biotechnology