SK3520 Experimental Methods in Molecular Biophysics 8.0 credits

Experimentella metoder i molekylär biofysik

  • Educational level

    Third cycle
  • Academic level (A-D)

    D
  • Subject area

  • Grade scale

Information for research students about course offerings

Start date: 2015 week 36

Intended learning outcomes

This course covers different experimental biophysical methods, how they are used to study structures and dynamics of biomolecules and their interactions. It also gives an overview of how these biophysical techniques are used in practice in biotechnology, drug development and in fundamental academic research. After this course the students are expected to be able to:

  • describe the main categories of biomolecules present in the human body, what their main functions are, and how they are built.
  • explain how interactions can take place between biomolecules and electromagnetic radiation
  • state what modern spectroscopic techniques that are used in molecular biophysics, and to explain the physical principles upon which these methods are based
  • Based on knowledge of the physical principles of the different biophysical techniques, judge and motivate which method(s) that is most appropriate to be applied to a particular biomolecular investigation.
  • Give an overview of how these methods are used in practice in biotechnology, drug development, clinical diagnostics and in fundamental academic research.
  • more specifically be able to judge what methods and in what way they can be applied/devleoped within the own area of research of the student.

Course main content

Fundamental properties of biomolecules. Basic thermodynamics of biomolecules, biomolecular dynamics and interactions. The principles of the following methods: Infrared-, Fluorescence-, Nuclear Magnetic Resonance-, Electron Spin Resonance-, Circular Dichroism- and Raman-spectroscopy, Mass spectrometry, X-ray crystallography, Electron Microscopy, Surface Plasmon Resonance, Atomic Force Microscopy. An overview of applications of these techniques in fundamental academic research, in pharmaceutical and biotech industry, and for clinical diagnostics, and morespecifically within own area of research.

Disposition

Lectures (34h), laborations/excercises (8h), project task, control tests, study visit (6h). Oral presentation of how method(s) presented in the course can be applied within own area of research.

Eligibility

Admitted to PhD studies in Physics, Biological Physics, or related fields of study.
Course given in English, if not all students would prefer Swedish.

Literature

Serdyuk IN et al, Methods in Molecular Biophysics, Cambridge Univ Press

Kurslitteratur: aktuell upplaga anslås på kursens hemsida senast fyra veckor innan kursstart.
Vetenskapliga artiklar.
Laborationsinstruktioner.

Examination

Requirements for final grade

One written examination (TEN1, 4hp, grades P/F), two oral project presentations (PRO1; 2hp, grades P/F), laborations, exercises and study visit (LAB1; 2hp, grades P/F)

Offered by

SCI/Applied Physics

Contact

Professor Jerker Widengren, jerker@biomolphysics.kth.se

Examiner

Jerker Widengren <jwideng@kth.se>

Version

Course syllabus valid from: Autumn 2014.