This course focuses on the exploitation of microorganisms and eukaryotic cellular systems and their biochemical pathways for the synthesis and modification of products at an industrial scale. Het is de interface tussen biochemie, microbiologie, eukaryote celbiologie en schaal-up / industriële bioprocessen. It exploits the basic knowledge from the Biochemistry and Microbiology courses from the second year and serves as a basis for the "Energy and Environment" and "Bioprocess Design" courses proposed at the end of the 4th year and during the 5th year.
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Prokaryotic and eukaryotic cell structure, including plant and mammalian cells, and cellular compartment functions, including sub-cellular localization of specific metabolic pathways
Specific prokaryotic and eukaryotic pathways for the production of complex biomolecules, including cellulose, alginate, chitins, glycoproteins and fatty acids, etc.
Describe prokaryotic and eukaryotic cell factories, including special features of eukaryotic hosts, such as codon usage, post-translational modifications, protein folding, protein processing, of disulfide bond formation
Metabolic engineering of fungi and plant systems, including transformation system (e.g. plastid targeting, Agrobacterium)- emphasis on carbohydrate polymers, plasticizer, lipid-derivatives, and punctually on biofuel production
Exploitation on other cell factory systems, such as algal system, and their metabolic pathways for the bioproduction.
Describe new technologies in synthetic biology, and how this has impact in bioproduction
Cost-benefit analysis (including environmental and social economical impact) for the sustainable production in the pathways specific/certain types of organisms which the products have potential applications
Process of mammalian cell-based system (e.g. Chinese Hamster Ovary cells, HEK293) for the manufacturing of biologics Development of mammalian cell-based processes, fed-batch and perfusion, aimed at commercial production for the production of biopharmaceuticals including scale-up aspects, requirements of patient safety and compliance to regulatory constraints
Demonstration of fed-batch bioreactor process using Chinese Hamster Ovary cell for the production of monoclonal antibody
Intended learning outcomes *
On completion of the course (ILOs), the students should be able to:
Describe and differentiate microorganisms and eukaryotic cellular systems, including mammalian cells, and their biochemical pathways.
Discuss with reference to scientific literature the challenges and potential opportunities in using genetically engineered cell factories for the production of biopharmaceuticals/biomolecules/chemicals/fuels at an industry scale.
Design a cell factory approach to the production of a given product, with reference to supporting scientific literature.
Design a cultivation process using mammalian cell system for the production of biologics fit for industrial purpose, i.e. robust and reproducible process using appropriate mammalian cell line and expression system, integrating scale-up constraints, Good Manufacturing Practice (GMP), and awareness of patient safety, as well as describe and discuss how such a process is developed
Design, evaluate, execute and present in written form an independent laboratory project report.