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FCB3206 Mammalian Cell Technology 6.0 credits

The course provides knowledge and skills on the cultivation of mammalian cells and its application for the production of biological drugs (biopharmaceuticals).

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Choose semester and course offering to see information from the correct course syllabus and course offering.

Headings with content from the Course syllabus FCB3206 (Spring 2022–) are denoted with an asterisk ( )

Content and learning outcomes

Course contents

The course teaches theory and methodology in the field of cell technology applied to animal cells.

Theoretical concepts included

  • basic techniques for animal cell culture in shake flasks and small-scale bioreactors
  • description and use of stable expression systems (cell lines)
  • overview of animal cell metabolism during cultivation, and its application in process design
  • aspects concerning commercial production such as patient safety and regulatory compliance
  • cells as products
  • application of knowledge from the course to develop and design culture processes

The student also receives practical training in

  • developing a process flow chart and strategy for cell culturing
  • choosing an appropriate cell line and expression system
  • developing a culture process for industrial application (scale-up constraints)
  • understanding the concept of "Good Manufacturing Practice" (GMP)
  • deciding on patient safety level and from that design a suitable process

Intended learning outcomes

Upon completion of the course, the course participant is expected to be able to

  • demonstrate knowledge of key theoretical concepts and basic methodology regarding the cultivation process of animal cells, as well as skills in communicating, reviewing, analyzing, and discussing the course topics (ÖVN1)
  • demonstrate the ability to use acquired knowledge to independently design, motivate and present strategies for animal cell culture process in order to manufacture a therapeutic biologics (PRO1)
  • demonstrate a basic understanding of the possibilities and limitations of the application of animal cell-culture techniques for the development of therapeutic biologics, and reflect on their use in a sustainable and ethical perspective  (PRO1)

Course disposition

The course comprises approximately 160 full-time study hours. The course content includes lectures with associated individual homework where the student reads and reviews relevant scientific articles, and summarizes the knowledge in the form of written assignments, and actively participates in discussions with other students.The course includes a project consisting of independently designing and presenting a strategy for animal cell culturing for the purpose of manufacturing a protein-based biological drug, which is communicated and examined in the form of a project report. The course also provides practical training in developing a cultivation process by the use of computer simulations, so-called virtual cultivation.

Literature and preparations

Specific prerequisites

Eligible for studies at the third-cycle level.

Recommended prerequisites

No information inserted

Equipment

No information inserted

Literature

  • Articles and additional material announced at course start
  • Butler M ed. (2007) “Cell Culture and Upstream Processing” Taylor and Francis ISBN 0-415-39969-6
  • Ozturk S and Hu W-S (2006) “Cell Culture Technology for Pharmaceutical and Cell-based Therapies” Taylor and Francis ISBN 0-8247-5334-8

Examination and completion

If the course is discontinued, students may request to be examined during the following two academic years.

Grading scale

P, F

Examination

  • PRO1 - Project, 4,0 hp, betygsskala: P, F
  • ÖVN1 - Exercises, 2,0 hp, betygsskala: P, F

Based on recommendation from KTH’s coordinator for disabilities, the examiner will decide how to adapt an examination for students with documented disability.

The examiner may apply another examination format when re-examining individual students.

Other information about the course and grade criteria are found in the course memo.

Opportunity to complete the requirements via supplementary examination

No information inserted

Opportunity to raise an approved grade via renewed examination

No information inserted

Examiner

Profile picture Antonius Van Maris

Ethical approach

  • All members of a group are responsible for the group's work.
  • In any assessment, every student shall honestly disclose any help received and sources used.
  • In an oral assessment, every student shall be able to present and answer questions about the entire assignment and solution.

Further information

Course web

Further information about the course can be found on the Course web at the link below. Information on the Course web will later be moved to this site.

Course web FCB3206

Offered by

CBH/Industrial Biotechnology

Main field of study

No information inserted

Education cycle

Third cycle

Add-on studies

No information inserted

Contact

Veronique Chotteau, veronique.chotteau@biotech.kth.se

Transitional regulations

If the examination form is changed, the student will be examined according to the examination form that applied when the student was admitted to the course. If the course is completed, the student is given the opportunity to be examined on the course for another two academic years.

Supplementary information

The course replaces FBB3650.

Additional regulations

Five students is the minimum number of participants for a course offering to be given.

Postgraduate course

Postgraduate courses at CBH/Industrial Biotechnology