BB1030 Microbiology 9.0 credits

Lectures

The course consists of 16 lectures with specified learning outcomes.

Labs

The course begins with light and phase contrast microscopy, general sterilization techniques and measurements of bacterial concentration. Next comes a connected, coherent lab which consists of planning and execution: isolation, clean cultivation, and characterization of bacteria from a natural environment with the help of microscopy, cultivation on selective and differentiated mediums, proof by enzymes and other microbial products along with antibiotic spectrum. Also, in this lab, nourishment mediums and substrates are to be prepared to cultivate aerobically and anaerobically as well as searching for information about isolated bacteria being cultivated. The results are presented in a seminar orally,

in a written report, and as a poster. A modern technique to color bacteria’s ribosome with the help of fluorescent-labeled probes is taught. This technique, FISH, is used to see specific bacteria in their natural environment. With this, 16s rRNA is isolated and sequenced for further work with the bioinformatics course.

Practice assignments

During the exercises, the students work with questions where the serach information online, in scientific literature and in the course literature. 

Group exercise

Students will work in groups to write an in-depth work around a topic where microorganisms play a fundamental role in creating a sustainable society.

The microbiology course deals with microorganisms, i.e. bacteria, viruses, protozoa, algae, and fungi that occur naturally in the body and in the environment, as well as those that we use as tools for various processes (in medicine, food production, technology, etc.). You will, for example, learn why fruits and berries keep better in sugar solution, what resistant bacteria actually are, and how biological water purification works. In the course, you will study the taxonomy, morphology, and physiology of microorganisms. A major focus is placed on the bacterial cell, its genetics, nutritional requirements, and growth, as well as how sterile techniques, disinfection, and antibiotics work to control growth. In the section on infectious diseases, you will learn about disease mechanisms such as toxins and how bacteria avoid the body’s immune response.

• Describe our common microorganisms with respect to:
• structure and growth
• occurrence in nature
• identification techniques
• basic taxonomy
• interactions and harmful effects on other living organisms
• Select and apply methods for controlling microbial growth
• Describe examples of how microbial activity affects the environment, humans, and society
• Give an in-depth description of societal factors that have led to widespread development of antimicrobial resistance and strategies to counteract this
• Give an in-depth explanation of how microorganisms are used as tools and producers in science and industry

CB1040 Introduction to Biotechnology, BB1050 Biotechnology or BB1150 Biochemistry 1

• TENB - Written exam, 5.5 credits, grading scale: A, B, C, D, E, FX, F
• PROA - Advanced study, 0.5 credits, grading scale: P, F
• LABA - Laboratory Work, 3.0 credits, grading scale: P, F

Based on recommendation from KTH’s coordinator for disabilities, the examiner will decide how to adapt an examination for students with documented disability.

The examiner may apply another examination format when re-examining individual students.

If the course is discontinued, students may request to be examined during the following two academic years.

Passed examination (TENA; 5,5 credits, grading scale A-F), passed laboratory exercises (LABA; 3 credits, grading scale Pass/Fail) and passed group exercise (PROA; 0.5 credits, grading scale Pass/Fail)

Paul Hudson

• All members of a group are responsible for the group's work.
• In any assessment, every student shall honestly disclose any help received and sources used.
• In an oral assessment, every student shall be able to present and answer questions about the entire assignment and solution.

Students are required to sign up at least two weeks in advance for examination.

The course is given provided at least seven students are admitted.