The course handles micro-organism morphology and structure along with their occurrence in nature. Taxonomical and physiological aspects of viruses, bacteria, protozoon, algae and fungi are covered. Special consideration is given to the bacteria cell, its nourishment demands and growth. Control of growth is clarified through sterilization, disinfection and antibiotics. The basics of bacterial genetics are covered along with bacterial genetic development toward modern phylogenetics with the help of gene sequencing of 16s rRNA. Mechanisms for sickness, for example: toxins and bacteria which are resistant against the body’s defenses, are also covered. Some meaningful microbial sicknesses are given as examples. Significant microbial processes for application within traditional and modern biotechnology are also covered. A couple of examples of such processes are biological water purification and ground-decontamination.
Intended learning outcomes *
After passing the course, the student should:
have a general knowledge about micro-organisms and their occurrence in nature
understand micro-organism replication and physiology
have an awareness of micro-organism cooperation with and ability to harm other living organisms
understand micro-organism implications for science and industry
master presentation techniques to be able convey scientific observations
Labs The course begins with light and phase contrast microscopy, general sterilization techniques and measurements of bacterial concentration. Next comes a connected, coherent lab which consists of planning and execution: isolation, clean cultivation, and characterization of bacteria from a natural environment with the help of microscopy, cultivation of selective and differentiated mediums, proof by enzymes and other microbial products along with antibiotic spectrums. Also, in this lab, nourishment mediums and substrates are to be prepared to cultivate aerobically and anaerobically as well as searching for information about isolated bacteria being cultivated. The results are presented in a seminar orally, in a written report, and as a poster. In a virus lab, differences and similarities between bacterial viruses and animal viruses are shown. The influenza virus is shown using the help of fluorescent microscopy. A modern technique to color bacteria’s ribosome with the help of fluorescent-labeled probes is taught. This technique, FISH, is used to see specific bacteria in their natural environment. With this, 16s rRna is isolated and sequenced for further work with the bioinformatics course.
Practice Assignments During the exercises, the students are provided with knowledge of how to search for information in the library using different search engines. The students will learn and utilize presentation techniques into two of the exercises. As a conclusion, the students may choose a microbial-related inquiry, search for information about it, write a paper (around 5 pages) and present it orally for the other students.
Literature and preparations
Specific prerequisites *
Completed upper secondary education including documented proficiency in English corresponding to English A.
The upper-secondary school before 1 July 2011 and adult education at upper-secondary level before 1 July 2012
Specific entry requirements: mathematics E, physics B and chemistry A. The grade Passed or 3 inn each of the subjects is required .
The upper-secondary school from 1 July 2011 and adult education at upper-secondary level from 1 July 2012 (Gy2011)
Specific entry requirements: Physics 2, Chemistry 1 and Mathematics 4. Minimum requirement is a pass grade.
BB1010 An Introduction to Biochemistry and BB1020 Cellbiology with immunology.
No information inserted
Brock Biology of Microorganisms, 14th edition, 2015. M. Madigan, J. Martinko, K. Bernder, D. Buckley, and D. Stahl.
Examination and completion
Grading scale *
A, B, C, D, E, FX, F
Grading scale: P, F
Grading scale: A, B, C, D, E, FX, F
Based on recommendation from KTH’s coordinator for disabilities, the examiner will decide how to adapt an examination for students with documented disability.
The examiner may apply another examination format when re-examining individual students.