BB1130 Analysis and Purification of Biomolecules 7.0 credits

Analys och rening av biomolekyler

Offering and execution

Course offering missing for current semester as well as for previous and coming semesters

Course information

Content and learning outcomes

Course contents *

This course is focused on the methods with constitute the foundation for purification and analysis of biological macro molecules, such as nucleic acids and proteins.  The techniques which will be discussed often all exist within both biochemical/biotechnological research and industry.  During the course these methods will be described and discussed during the lectures, labs and a smaller project.  The course book will be complemented with descriptions from other literature and with relevant case studies from research.  If the opportunity arises, a visit to a company will be arranged.  In order for you to be able to understand and handle problems both in large and small scales, differences between analytical and large-scale problem inquiries will be illustrated.  Guest lecturer with much insight into those specific techniques will be invited in order raise the quality of the course.

Intended learning outcomes *

After passing the course, the student should be able to:

  • Explain different protein separation methods, their properties, advantages and disadvantages and when it is most appropriate to use them.
  • Explain the different analysis methods reviewed during the course, their properties, advantages and disadvantages
  • Plan a working cleaning procedure for a protein with consideration to what has been learned in the course.
  • Plan characterization of the purified protein and be able to take into consideration the available methods’ advantages and restrictions
  • Carry out a lab which includes many of those reviewed techniques
  • calculate gains/losses in a purification process and suggest improvements in order to reduce losses

 Write a report which sheds light on the methods used and interpret data from the purification/analyses done

Course Disposition

No information inserted

Literature and preparations

Specific prerequisites *

No information inserted

Recommended prerequisites

BB1010 Introduction to Biotechnology
or
BB1050 Biotechnology and BB1080 Biochemistry, Theory
or 
BB1090 Biochemistry, Theory 
or
BB2050 Biochemistry

Equipment

No information inserted

Literature

Information about litterature will be given athttps://www.kth.se/social/course/BB1130/ 

at least three weeks prior to the start of the course

Examination and completion

Grading scale *

A, B, C, D, E, FX, F

Examination *

  • LAB1 - Laboratory Work, 3.0 credits, Grading scale: A, B, C, D, E, FX, F
  • TEN1 - Examination, 4.0 credits, Grading scale: A, B, C, D, E, FX, F

Based on recommendation from KTH’s coordinator for disabilities, the examiner will decide how to adapt an examination for students with documented disability.

The examiner may apply another examination format when re-examining individual students.

Other requirements for final grade *

A written exam (TENA; 4 credits, grading scale A-F), Lab course (LABA; 3 credits, grading scale Pass/Fail).

Opportunity to complete the requirements via supplementary examination

No information inserted

Opportunity to raise an approved grade via renewed examination

No information inserted

Examiner

Sophia Hober

Further information

Course web

Further information about the course can be found on the Course web at the link below. Information on the Course web will later be moved to this site.

Course web BB1130

Offered by

BIO/Biotechnology

Main field of study *

Biotechnology, Technology

Education cycle *

First cycle

Add-on studies

No information inserted

Contact

Jenny Ottosson Takanen, jennyot@kth.se

Ethical approach *

  • All members of a group are responsible for the group's work.
  • In any assessment, every student shall honestly disclose any help received and sources used.
  • In an oral assessment, every student shall be able to present and answer questions about the entire assignment and solution.

Supplementary information

Students are required to sign up at least two weeks in advance for examination.

The course is given provided at least seven students are admitted.

Overlaps with BB1210-Purification of Biomolecules