BB1200 Analysis of Biomolecules 6.0 credits

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Content and learning outcomes
Course contents
The course gives a general knowledge of methods and technologies for the analysis of biomolecules. The main focus is on analysis of proteins, but also analysis of other biomolecules such as DNA and RNA is included. Basic experimental design will also be included. The course contains both theoretical and practical parts.
The following analytical methods will be treated in the course:
- Electrophoresis
- Spectrophotometric methods
- Immunochemical and immunotechnological methods
- Mass spectroscopy
- Microscopy
- Structural biology methods
- Biosensor methods
- Radiochemical methods
- Flow cytometry
- Analytical chromatography
Prerequisites equivalent to BB1030 Microbiology, BB1210 Purification of biomolecules and BB1190 Genetechnology are recommended.
Intended learning outcomes
After completion of the course the student shall have
Knowledge and understanding to:
- Describe and explain common analytical methods for biomolecules (TEN1, SEM1)
- Apply methods for analysis of biomolecules (LAB1)
Skills and abilities to:
- Report orally and in writing within the subject. (LAB1, SEM1)
- Choose and combine appropriate methods and technologies for given questions. (SEM1, LAB1)
- Design experiments (LAB1, SEM1)
Values and approaches to:
- Critically discuss possibilities and limitations with the technologies that are treated in the course. (TEN1, LAB1, SEM1)
- Review and give constructive feedback on oral and written reports within the subject. (SEM1)
Course disposition
Literature and preparations
Specific prerequisites
Completed course BB1150 Biochemistry 1.
Recommended prerequisites
BB1150 Biochemistry, BB1030 Microbiology, BB1190 Gene Technology, BB1210 Purification of Biomolecules or equivalent courses.
Equipment
Literature
Examination and completion
If the course is discontinued, students may request to be examined during the following two academic years.
Grading scale
Examination
- LAB1 - Laboratory Work, 2.0 credits, grading scale: P, F
- SEM1 - Seminar, 2.0 credits, grading scale: P, F
- TEN1 - Written exam, 2.0 credits, grading scale: A, B, C, D, E, FX, F
Based on recommendation from KTH’s coordinator for disabilities, the examiner will decide how to adapt an examination for students with documented disability.
The examiner may apply another examination format when re-examining individual students.
Opportunity to complete the requirements via supplementary examination
Opportunity to raise an approved grade via renewed examination
Examiner
Ethical approach
- All members of a group are responsible for the group's work.
- In any assessment, every student shall honestly disclose any help received and sources used.
- In an oral assessment, every student shall be able to present and answer questions about the entire assignment and solution.
Further information
Course web
Further information about the course can be found on the Course web at the link below. Information on the Course web will later be moved to this site.
Course web BB1200Offered by
Main field of study
Education cycle
Add-on studies
Supplementary information
Overlaps with the courses BB1100, BB1105 and BB1130