BB2485 Metabolic Engineering 7.5 credits

Metabolic Engineering

  • Education cycle

    Second cycle
  • Main field of study

    Biotechnology
  • Grading scale

    A, B, C, D, E, FX, F

Course offerings

Spring 19 for programme students

Spring 20 for programme students

Intended learning outcomes

After completing the course, students should be able to:

  • Quantitatively describe metabolic pathways for production of alcohols, organic acids, amino acids, monomers, and polymers.
  • Characterize the above pathways, discuss their engineering requirements, and propose relevant metabolic engineering strategies.
  • Perform metabolic pathway balancing in regard to redox, elemental, and ATP balance.
  • Use metabolic control analysis to identify important steps in pathway control.
  • Construct a mathematical representation of a metabolic network, and calculate the internal fluxes based on provided external measurements.
  • Design metabolic engineering strategies for product formation using genome-scale metabolic models.

Describe the latest developments in genome-scale metabolic modelling.

Course main content

  • Metabolic pathways for production of organic acids, amino acids, alcohols, monomers, and polymers.
  • The underlying concept behind balancing the above pathways, based on elemental, redox and energy balance.
  • Metabolic control analysis.
  • Metabolic engineering strategies.
  • Metabolic flux analysis (MFA).
  • The concepts of genome-scale stoichiometric metabolic models.
  • The use of genome-scale models for designing metabolic engineering strategies.
  • The inclusion of thermodynamic constraints in genome-scale stoichiometric models.
  • State-of-the-art genome-scale modelling, combining stoichiometry, proteomics and metabolomics.

Eligibility

20 ECTS in biochemistry, microbiology and gene technology/molecular biology; 20 ECTS in chemistry; 20 ECTS in mathematics/numerical analysis/computer science

Literature

Course literature will be announced on the course homepage 4 weeks prior to course start. Previous versions of the course has used "Biofuels and Green chemicals" by Prof. Gen Larsson, as well as peer-reviewed scientific articles.

Examination

  • PRO1 - Assignment in metabolic modelling, 2.5, grading scale: P, F
  • TEN1 - Examination, 5.0, grading scale: A, B, C, D, E, FX, F

Offered by

CBH/Industrial Biotechnology

Examiner

Antonius Van Maris <tonvm@kth.se>

Version

Course syllabus valid from: Spring 2019.
Examination information valid from: Spring 2019.