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Before choosing courseCB2010 Downstream processing of biological products 7.5 creditsAdministrate About course

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* Retrieved from Course syllabus CB2010 (Autumn 2019–)

Content and learning outcomes

Course contents

An important task for bioengineers is to design and execute efficient and sustainable downstream processes to achieve a pure bioproduct. The desired purity and the biophysical characteristics of the product will have large effects on the design of the process. The course aims to give an understanding of the fundamentals of recovery/purification operations for biopharmaceutical production and how these operations are developed for use at production scale. Methods that are relevant for the down-stream process (DSP) will be described and discussed: different means for characterization of biological mixtures, methods used for separation of solid and soluble phases, technologies used to separate dissolved molecules from each other as well as methods utilized to concentrate the product. How the biophysical properties of the target molecule as well as the contaminating molecules can be exploited in the design of an effective DSP will be presented. The theoretical backgrounds of the methods will be described as well as their practical use, advantages and drawbacks. How demands on purity and yield of bio-products affect the design of the (DSP) will be reviewed. Furthermore, the different possible choices of unit operations for downstream purification methods and how the used upstream methods will affect those, will be discussed.

Examples of methods that will be discussed are: filtration, sedimentation, precipitation, centrifugation, extraction, crystallization and chromatography.

Intended learning outcomes

  • Describe, compare and understand a set of general methods used for downstream processing in large scale processing.
  • Describe and explain the tasks a given unit operation can solve in a downstream process.
  • Describe and motivate a set of general analytical tools used to track the downstream process.

Course Disposition

No information inserted

Literature and preparations

Specific prerequisites

Pass on the courses below:

BB1200 Analysis of Biomolecules 6.0 credits
BB1210 Purification of Biomolecules 6.0 credits

Recommended prerequisites

No information inserted

Equipment

No information inserted

Literature

No information inserted

Examination and completion

If the course is discontinued, students may request to be examined during the following two academic years.

Grading scale

A, B, C, D, E, FX, F

Examination

  • PRO1 - Project, 2,0 hp, betygsskala: P, F
  • TEN1 - Written exam, 5,5 hp, betygsskala: A, B, C, D, E, FX, F

Based on recommendation from KTH’s coordinator for disabilities, the examiner will decide how to adapt an examination for students with documented disability.

The examiner may apply another examination format when re-examining individual students.

Other requirements for final grade

Project (PROJ1; 2,0 credits, grading scale P/F)
Examination (TEN 1; 5,5 credits, grading scale A - F)

Opportunity to complete the requirements via supplementary examination

No information inserted

Opportunity to raise an approved grade via renewed examination

No information inserted

Examiner

Profile picture Sophia Hober

Ethical approach

  • All members of a group are responsible for the group's work.
  • In any assessment, every student shall honestly disclose any help received and sources used.
  • In an oral assessment, every student shall be able to present and answer questions about the entire assignment and solution.

Further information

Course web

Further information about the course can be found on the Course web at the link below. Information on the Course web will later be moved to this site.

Course web CB2010

Offered by

CBH/Industrial Biotechnology

Main field of study

Biotechnology

Education cycle

Second cycle

Add-on studies

No information inserted