• Introduction to processes for the manufacturing of biopharmaceuticals. • Introduction to animal cell based cultivation process and relations/interactions between cultivation and other steps of the biopharmaceutical process.
• Usage of stable expression systems and description of the existing systems, e.g. cell lines.
• Review of cell metabolism relevant for the animal cell cultivation and application to process design. Scale-up aspects.
• Issues of commercial production, e.g. requirements of patient safety and compliance to regulatory constraints.
• Integration of the different course materials into development and design of a cultivation process.
• Cells as product
• Basic techniques for mammalian cell culture in shake flask, and small-scale fed-batch bioreactor operations
FBB3650 Animal Cell Technology 7.5 credits
This course has been discontinued.
Last planned examination: Spring 2024
Decision to discontinue this course:
No information inserted
Information for research students about course offerings
Contact the examiner for information.
Content and learning outcomes
Course contents
Intended learning outcomes
The students will learn how to design a cultivation process to produce a biopharmaceutical drug protein. In particular, after the course, they will be able to
• design a cultivation process flow sheet of operations
• select an appropriate cell line
• choose an expression system
• design a cultivation process fit for industrial purpose, i.e. integrating scale-up constraints, Good Manufacturing Practice (GMP), manufacturing operation
• select the level of safety required for the patients and design a process accordingly
• be able to design a strategy to develop a cultivation process
Literature and preparations
Specific prerequisites
The KTH course
BB1120 Cultivation technology or
BB2080 Bioprocess Technology or equivalent.
Recommended prerequisites
Equipment
Literature
Examination and completion
If the course is discontinued, students may request to be examined during the following two academic years.
Grading scale
Examination
- DEL1 - Participation, 2.5 credits, grading scale: P, F
- PRO1 - Project work, 5.0 credits, grading scale: P, F
Based on recommendation from KTH’s coordinator for disabilities, the examiner will decide how to adapt an examination for students with documented disability.
The examiner may apply another examination format when re-examining individual students.
Other requirements for final grade
(1) > 50 % attendance at lectures, and
(2) Score > 60 % of max points at written examination, and
(3) > 80 % of home assignments approved
Opportunity to complete the requirements via supplementary examination
Opportunity to raise an approved grade via renewed examination
Examiner
Ethical approach
- All members of a group are responsible for the group's work.
- In any assessment, every student shall honestly disclose any help received and sources used.
- In an oral assessment, every student shall be able to present and answer questions about the entire assignment and solution.