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KD2330 Analytical Separations 7.5 credits

Advanced course with focus on separation techniques and mass spectrometry. The course provides theoretical knowledge of different techniques and practical training in a research-based laboratory project.

About course offering

For course offering

Spring 2025 Start 14 Jan 2025 programme students

Target group

Open for all students

Part of programme

Master of Science in Engineering and in Education, åk 4, MAKE, Conditionally Elective

Master of Science in Engineering and in Education, åk 5, MAKE, Conditionally Elective

Master's Programme, Molecular Science and Engineering, åk 1, Conditionally Elective


P3 (7.5 hp)


14 Jan 2025
16 Mar 2025

Pace of study


Form of study

Normal Daytime

Language of instruction


Course location

KTH Campus

Number of places

Min: 12

Planned modular schedule


For course offering

Spring 2025 Start 14 Jan 2025 programme students

Application code



For course offering

Spring 2025 Start 14 Jan 2025 programme students


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Course coordinator

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Headings with content from the Course syllabus KD2330 (Autumn 2021–) are denoted with an asterisk ( )

Content and learning outcomes

Course contents

The lectures discuss basic principles of different separation techniques and give a summary of a few of the most important methods like capillary gas chromatography, HPLC, capillary electrophoresis and combinations of these with mass spectrometry. In this connection, specific instrumental aspects, sample workup methods, optimizing of separations and problem solving, are discussed. When relevant the different methods, techniques and applications are also discussed regarding environmental and sustainability issues.

The laboratory course includes a group assignment or minor project, which is often related to an ongoing research project or a relevant industrial project.

Intended learning outcomes

After completion of the course the student will be able to:

  • Explain the basic principles for gas chromatography, liquid chromatography, capillary electrophoresis and mass spectrometry, describe the different methods, which are used in each technique respectively and the instrumental variants that exist.
  • Suggest and explain how the method development and the optimization can be accomplished for the different techniques.
  • Participate actively in the demonstration laboratory exercises.
  • Define and compare the different methods, choose technique/method for specific analysis problems and motivate the choices also with respect to environmental and sustainability aspects.
  • Plan, perform and evaluate a laboration project in a group, present the lab project in a written report and an oral presentation for the other students in the course.

Literature and preparations

Specific prerequisites

At least 150 credits from grades 1, 2 and 3 of which at least 110 credits from years 1 and 2, and bachelor's work must be completed, within a programme that includes:
75 university credits (hp) in chemistry or chemical engineering, 20 university credits (hp) in mathematics and 6 university credits (hp) in computer science or corresponding.

Recommended prerequisites

Recommended prior knowledge:
KD1280/KD1290 Chemical Analysis or corresponding knowledge.


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Quantitative Chemical Analysis, D.C. Harris, C.A. Lucy, 10ed ISBN: 978-1-319-32450-6

Examination and completion

If the course is discontinued, students may request to be examined during the following two academic years.

Grading scale

A, B, C, D, E, FX, F


  • LAB1 - Laboratory Work, 4.5 credits, grading scale: P, F
  • TEN1 - Oral exam, 3.0 credits, grading scale: A, B, C, D, E, FX, F

Based on recommendation from KTH’s coordinator for disabilities, the examiner will decide how to adapt an examination for students with documented disability.

The examiner may apply another examination format when re-examining individual students.

Other requirements for final grade

Final grade for the complete course is based on the grade of TEN1.

Opportunity to complete the requirements via supplementary examination

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Opportunity to raise an approved grade via renewed examination

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Ethical approach

  • All members of a group are responsible for the group's work.
  • In any assessment, every student shall honestly disclose any help received and sources used.
  • In an oral assessment, every student shall be able to present and answer questions about the entire assignment and solution.

Further information

Course room in Canvas

Registered students find further information about the implementation of the course in the course room in Canvas. A link to the course room can be found under the tab Studies in the Personal menu at the start of the course.

Offered by

Main field of study

Chemical Science and Engineering, Chemistry and Chemical Engineering

Education cycle

Second cycle

Add-on studies

No information inserted

Supplementary information

The course has the same content as the courses KD2020 Organic and Biochemical Analytical Separationsand KD2030 Analytical Separation Methods.

Only one of the courses KD2330, KD2020 and KD2030 may be included in the exam.