Introduction to fluorescence, physical description of absorption and emission processes, fluorescence markers and their characteristics, environmental effects / fluorescent molecular sensors, other photoinduced non-fluorescent states of fluorophores, polarization and molecularr otational measurements, resonant energy transfer (FRET) and molecular distance measurements with fluorescence, ultra-sensitive fluorescence spectroscopic and fluorescence microscopic techniques, including single-molecule spectroscopy methods and fluctuation spectroscopy, fluorescence spectroscopic applications in biology, medicine and drug development.
SK2521 Fluorescence Spectroscopy for Biomolecular Studies 6.0 credits
This course has been discontinued.
Last planned examination: Autumn 2022
Decision to discontinue this course:
No information insertedInformation per course offering
Course offerings are missing for current or upcoming semesters.
Course syllabus as PDF
Please note: all information from the Course syllabus is available on this page in an accessible format.
Course syllabus SK2521 (Spring 2022–)Content and learning outcomes
Course contents
Intended learning outcomes
After completing the course the student should be able to:
- explain the basic physical mechanisms of fluorescence.
- explain how the interaction between biomolecules and electromagnetic radiation, as well as environmental effects, can generate differences in the measured fluorescence parameters, and how these differences can be utilized for the observation of biomolecules and their interaction.
- explain the physical principles of the most important fluorescence techniques in the biomedical research field, and what kind of questions these techniques can be used to answer.
- describe and motivate which factors limit the performance of fluorescence techniques, and how measurement results obtained are evaluated.
- describe, discuss and assess the potential of key elements in the latest developments in the fluorescence spectroscopic field.
Literature and preparations
Specific prerequisites
Mathematics corresponding to B2, D2, E2, F2, M2, T2. Fundamental knowledge of Physics, Course SK2520 (Experimental methods in molecular biophysics).
English B / English 6
Recommended prerequisites
Fundamental knowledge in quantum mechanics and optics advantageous, but not absolutely required.
Equipment
Literature
Examination and completion
If the course is discontinued, students may request to be examined during the following two academic years.
Grading scale
Examination
- LAB1 - Laboratory Work, 1.0 credits, grading scale: P, F
- PRO1 - Project, 1.0 credits, grading scale: P, F
- TEN1 - Examination, 4.0 credits, grading scale: A, B, C, D, E, FX, F
Based on recommendation from KTH’s coordinator for disabilities, the examiner will decide how to adapt an examination for students with documented disability.
The examiner may apply another examination format when re-examining individual students.
One written examination (TEN1, 4hp, grades A-F), one oral project presentation (PRO1; 1hp, grades P/F), laborations, exercises (LAB1; 1hp, grades P/F)
Opportunity to complete the requirements via supplementary examination
Opportunity to raise an approved grade via renewed examination
Yes
Examiner
Ethical approach
- All members of a group are responsible for the group's work.
- In any assessment, every student shall honestly disclose any help received and sources used.
- In an oral assessment, every student shall be able to present and answer questions about the entire assignment and solution.
Further information
Course room in Canvas
Offered by
Main field of study
Education cycle
Add-on studies
Contact
Supplementary information
The course is given 2018, 2020, 2022 etc.