Skip to main content
Till KTH:s startsida Till KTH:s startsida

SK2533 Experimental Biophysics 7.5 credits

The course gives an introduction to biomolecules, their structures, dynamics and interactions, and how experimental biophysical techniques can be used to investigate biomolecular properties. The course also gives an overview of how these techniques are used in practice within biotechnology, drug development, and academic research.

About course offering

For course offering

Autumn 2024 Start 28 Oct 2024 programme students

Target group

No information inserted

Part of programme

Master's Programme, Engineering Physics, åk 1, TFYE, Conditionally Elective


P2 (7.5 hp)


28 Oct 2024
13 Jan 2025

Pace of study


Form of study

Normal Daytime

Language of instruction


Course location


Number of places

Places are not limited

Planned modular schedule


For course offering

Autumn 2024 Start 28 Oct 2024 programme students

Application code



For course offering

Autumn 2024 Start 28 Oct 2024 programme students


Jerker Widengren


No information inserted

Course coordinator

No information inserted


No information inserted
Headings with content from the Course syllabus SK2533 (Spring 2022–) are denoted with an asterisk ( )

Content and learning outcomes

Course contents

Interaction between biomolecules and electromagnetic radiation. Principles of the most important methods of experimental biophysics, including IR, fluorescence, nuclear magnetic resonance, electron magnetic resonance, circular dichroism, and Raman spectroscopy, mass spectrometry, X-ray crystallography, electron microscopy, surface plasmon resonance, nuclear force microscopy, how these methods can be used to obtain information on different biomolecular properties and interactions, as well as their relative strengths and weaknesses. Overview of current development trends of these methods, as well as applications in academic research, pharmaceutical and biotechnology industries, and in healthcare.

Intended learning outcomes

The course provides good knowledge of the most important experimental methods in biophysics, their strengths and weaknesses, and how they are used in the field of biomedicine. The course is adapted for students with a background in engineering subjects.

After completing the course, students should be able to:

  • explain the principles of the main methods used in experimental biophysics.
  • compare strengths and weaknesses between these methods, and analyze and identify which methods are best suited to study different biomolecular properties or interactions, at the molecular, cellular, tissue or organism level.
  • explain and be able to evaluate the application potential of current development trends of these methods.

Literature and preparations

Specific prerequisites

Completed degree project at the undergraduate level in engineering physics or medical technology.

English B / English 6

Recommended prerequisites

No information inserted


No information inserted


No information inserted

Examination and completion

If the course is discontinued, students may request to be examined during the following two academic years.

Grading scale

A, B, C, D, E, FX, F


  • LAB1 - Laboratory work, 1.5 credits, grading scale: P, F
  • PRO1 - Project, 1.0 credits, grading scale: P, F
  • TEN1 - Written exam, 5.0 credits, grading scale: A, B, C, D, E, FX, F

Based on recommendation from KTH’s coordinator for disabilities, the examiner will decide how to adapt an examination for students with documented disability.

The examiner may apply another examination format when re-examining individual students.

Opportunity to complete the requirements via supplementary examination

No information inserted

Opportunity to raise an approved grade via renewed examination



Ethical approach

  • All members of a group are responsible for the group's work.
  • In any assessment, every student shall honestly disclose any help received and sources used.
  • In an oral assessment, every student shall be able to present and answer questions about the entire assignment and solution.

Further information

Course room in Canvas

Registered students find further information about the implementation of the course in the course room in Canvas. A link to the course room can be found under the tab Studies in the Personal menu at the start of the course.

Offered by

Main field of study

Engineering Physics

Education cycle

Second cycle

Add-on studies

No information inserted


Jerker Widengren