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SK2513 Cell Culture: Theory and Practice 4.0 credits

Course memo Spring 2021-1

Version 1 – 01/12/2021, 3:07:17 PM

Course offering

Spring 2021-1 (Start date 18/01/2021, English)

Language Of Instruction

English

Offered By

SCI/Applied Physics

Course memo Spring 2021-1

Course presentation

Content and learning outcomes

Course contents

Cell culture laboratory: equipment, appliances, consumables. Cell culture methods. Media and solutions. Contamination of cell cultures. Cell viability tests. Good cell culture practice. Ethical and sustainability issues related to the cell culture.

Intended learning outcomes

After completing the course the student should be able to:

  • independently subculture an animal cell line 
  • describe the equipment, appliances, and consumables needed for cell culture
  • account for the different aspects of good cell culture practice
  • perform calculations necessary for a cell line propagation and for preparation of stock solutions

Learning activities

Six Zoom meetings:

- one introductory

- five that correspond to the course lectures 1-5

Lectures will be pre-recorded and made available for the students one by one as the course proceeds.

During the meetings 2-6, we will discuss the material of the corresponding lecture and the students will write a mini-test on this material.

Due to Covid-19 situation, the practical labs in the course have to be performed on a later occasion. This will be arranged individually with each student.

 

Detailed plan

Learning activities Content Preparations
Meeting 1 Introduction to the course  
Meeting 2 Materials and equipment for cell culture Review Lecture 1
Meeting 3 Cell culture media Review Lecture 2
Meeting 4 Cell culture protocols Review Lecture 3
Meeting 5

Viability tests

Cell culture contamination

Review Lecture 4
Meeting 6 Good cell culture practice Review Lecture 5


Schema VT-2021-806 

Preparations before course start

Recommended prerequisites

SK2530 (Introduction to Biomedicine) or SK2531 (Biomedicine for Engineers) is recommended, but is not a requirement.

Literature

  • Lecture handouts
  • ECACC Handbook – Fundamental Techniques for ECACC Cell Lines
  • Technical Tip from Roche: Culture of Animal Cells - Basic Techniques
  • Tips and Tricks for Successful Cell Cultures

Support for students with disabilities

Students at KTH with a permanent disability can get support during studies from Funka:

Funka - compensatory support for students with disabilities

Please inform the course coordinator if you need compensatory support during the course. Present a certificate from Funka.

Examination and completion

Grading scale

A, B, C, D, E, FX, F

Examination

  • LAB1 - Laboratory experiments, 2.0 credits, Grading scale: P, F
  • TEN1 - Written exam, 2.0 credits, Grading scale: A, B, C, D, E, FX, F

Based on recommendation from KTH’s coordinator for disabilities, the examiner will decide how to adapt an examination for students with documented disability.

The examiner may apply another examination format when re-examining individual students.

(Section below is not part of the syllabus)

Laboratory experiments ( LAB1 )

Three lab sessions should be attended. During two of them, the student has to successfully perform cell subculture procedures, carrying out all needed calculations.

Written exam ( TEN1 )

Three-hour written exam in Zoom. Depending on the Covid-19 situation, the exam can be modified and supplemented by a short individual oral examination in Zoom.

Other requirements for final grade

Laboratory exercises (LAB1 2,0 credits, grade: P)

Written exam (TEN1 2,0 credits, grade scale: A, B, C, D, E)

Grading criteria/assessment criteria

For grade A, the student should have a very good knowledge and clear understanding of all the topics discussed during the course. The student should be fluent with calculations involved in cell culture and cell treatments.

For grade C, the student should have a good knowledge and understanding of the topics discussed during the course. The student should have a clear idea of how to perform the calculations involved in cell culture and cell treatments.

For grade E, the student should have a good knowledge and understanding of the most important parts of the topics discussed during the course. The student should have a general idea of how to perform the calculations involved in cell culture and cell treatments.

Ethical approach

  • All members of a group are responsible for the group's work.
  • In any assessment, every student shall honestly disclose any help received and sources used.
  • In an oral assessment, every student shall be able to present and answer questions about the entire assignment and solution.

Further information

No information inserted

Round Facts

Offered By

SCI/Applied Physics

Language Of Instruction

English

Course offering

Spring 2021-1 (Start date 18/01/2021, English)

Contacts