Cell culture laboratory: equipment, appliances, consumables. Cell culture methods. Media and solutions. Contamination of cell cultures. Cell viability tests. Good cell culture practice. Ethical and sustainability issues related to the cell culture.
Intended learning outcomes
After completing the course the student should be able to:
independently subculture an animal cell line
describe the equipment, appliances, and consumables needed for cell culture
account for the different aspects of good cell culture practice
perform calculations necessary for a cell line propagation and for preparation of stock solutions
Six Zoom meetings:
- one introductory
- five that correspond to the course lectures 1-5
Lectures will be pre-recorded and made available for the students one by one as the course proceeds.
During the meetings 2-6, we will discuss the material of the corresponding lecture and the students will write a mini-test on this material.
Due to Covid-19 situation, the practical labs in the course have to be performed on a later occasion. This will be arranged individually with each student.
Please inform the course coordinator if you need compensatory support during the course. Present a certificate from Funka.
Examination and completion
A, B, C, D, E, FX, F
LAB1 - Laboratory experiments, 2.0 credits, Grading scale: P, F
TEN1 - Written exam, 2.0 credits, Grading scale: A, B, C, D, E, FX, F
Based on recommendation from KTH’s coordinator for disabilities, the examiner will decide how to adapt an examination for students with documented disability.
The examiner may apply another examination format when re-examining individual students.
(Section below is not part of the syllabus)
Laboratory experiments ( LAB1 )
Three lab sessions should be attended. During two of them, the student has to successfully perform cell subculture procedures, carrying out all needed calculations.
Written exam ( TEN1 )
Three-hour written exam in Zoom. Depending on the Covid-19 situation, the exam can be modified and supplemented by a short individual oral examination in Zoom.
Other requirements for final grade
Laboratory exercises (LAB1 2,0 credits, grade: P)
Written exam (TEN1 2,0 credits, grade scale: A, B, C, D, E)
Grading criteria/assessment criteria
For grade A, the student should have a very good knowledge and clear understanding of all the topics discussed during the course. The student should be fluent with calculations involved in cell culture and cell treatments.
For grade C, the student should have a good knowledge and understanding of the topics discussed during the course. The student should have a clear idea of how to perform the calculations involved in cell culture and cell treatments.
For grade E, the student should have a good knowledge and understanding of the most important parts of the topics discussed during the course. The student should have a general idea of how to perform the calculations involved in cell culture and cell treatments.
All members of a group are responsible for the group's work.
In any assessment, every student shall honestly disclose any help received and sources used.
In an oral assessment, every student shall be able to present and answer questions about the entire assignment and solution.