Course contents

Cell culture laboratory: equipment, appliances, consumables. Cell culture methods. Media and solutions. Contamination of cell cultures. Cell viability tests. Good cell culture practice. Ethical and sustainability issues related to the cell culture.

Intended learning outcomes

After completing the course the student should be able to:

• independently subculture an animal cell line 
• describe the equipment, appliances, and consumables needed for cell culture
• account for the different aspects of good cell culture practice
• perform calculations necessary for a cell line propagation and for preparation of stock solutions

Course Disposition

No information inserted

Specific prerequisites

For single courses students, 120 hp is required in engineering and natural sciences or equivalent, as well as knowledge of English B or equivalent.

Recommended prerequisites

SK2530 (Introduction to Biomedicine) or SK2531 (Biomedicine for Engineers) is recommended, but is not a requirement.

Equipment

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Literature

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If the course is discontinued, students may request to be examined during the following two academic years.

Grading scale

A, B, C, D, E, FX, F

Examination

• LAB1 - Laboratory experiments, 2,0 hp, betygsskala: P, F
• TEN1 - Written exam, 2,0 hp, betygsskala: A, B, C, D, E, FX, F

Based on recommendation from KTH’s coordinator for disabilities, the examiner will decide how to adapt an examination for students with documented disability.

The examiner may apply another examination format when re-examining individual students.

Other requirements for final grade

Laboratory exercises (LAB1 2,0 credits, grade: P)

Written exam (TEN1 2,0 credits, grade scale: A, B, C, D, E)

Opportunity to complete the requirements via supplementary examination

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Opportunity to raise an approved grade via renewed examination

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Examiner

Marina Zelenina

Ethical approach

• All members of a group are responsible for the group's work.
• In any assessment, every student shall honestly disclose any help received and sources used.
• In an oral assessment, every student shall be able to present and answer questions about the entire assignment and solution.

Course web

Further information about the course can be found on the Course web at the link below. Information on the Course web will later be moved to this site.

Offered by

SCI/Applied Physics

Main field of study

Biotechnology

Education cycle

Second cycle

Add-on studies

No information inserted

Contact

Marina Zelenina (marinaz@kth.se)