SK2513 Cell Culture: Theory and Practice 4.0 credits
Content and learning outcomes
Cell culture laboratory: equipment, appliances, consumables. Cell culture methods. Media and solutions. Contamination of cell cultures. Cell viability tests. Good cell culture practice. Ethical and sustainability issues related to the cell culture.
Intended learning outcomes
After completing the course the student should be able to:
- independently subculture an animal cell line
- describe the equipment, appliances, and consumables needed for cell culture
- account for the different aspects of good cell culture practice
- perform calculations necessary for a cell line propagation and for preparation of stock solutions
Literature and preparations
For single courses students, 120 hp is required in engineering and natural sciences or equivalent, as well as knowledge of English B or equivalent.
SK2530 (Introduction to Biomedicine) or SK2531 (Biomedicine for Engineers) is recommended, but is not a requirement.
Examination and completion
If the course is discontinued, students may request to be examined during the following two academic years.
- LAB1 - Laboratory experiments, 2.0 credits, grading scale: P, F
- TEN1 - Written exam, 2.0 credits, grading scale: A, B, C, D, E, FX, F
Based on recommendation from KTH’s coordinator for disabilities, the examiner will decide how to adapt an examination for students with documented disability.
The examiner may apply another examination format when re-examining individual students.
Other requirements for final grade
Laboratory exercises (LAB1 2,0 credits, grade: P)
Written exam (TEN1 2,0 credits, grade scale: A, B, C, D, E)
Opportunity to complete the requirements via supplementary examination
Opportunity to raise an approved grade via renewed examination
- All members of a group are responsible for the group's work.
- In any assessment, every student shall honestly disclose any help received and sources used.
- In an oral assessment, every student shall be able to present and answer questions about the entire assignment and solution.
Further information about the course can be found on the Course web at the link below. Information on the Course web will later be moved to this site.Course web SK2513