BB2020 Molecular Enzymology 7.5 credits

Molekylär enzymologi

Please note

The information on this page is based on a course syllabus that is not yet valid.

  • Education cycle

    Second cycle
  • Main field of study

    Biotechnology
  • Grading scale

    A, B, C, D, E, FX, F

Course offerings

Autumn 19 for programme students

Autumn 18 for programme students

Autumn 18 Doktorand for single courses students

  • Periods

    Autumn 18 P2 (7.5 credits)

  • Application code

    10185

  • Start date

    29/10/2018

  • End date

    14/01/2019

  • Language of instruction

    English

  • Campus

    AlbaNova

  • Tutoring time

    Daytime

  • Form of study

    Normal

  • Number of places *

    Max. 1

    *) If there are more applicants than number of places selection will be made.

  • Course responsible

    Mats Martinell <matsm@kth.se>

  • Teacher

    Mats Martinell <matsm@kth.se>

  • Target group

    For doctoral students at KTH.

Intended learning outcomes

The course will give a fundamental understanding of enzyme function and will teach the methods that form the basis for enzyme characterisation.

Course main content

  • Catalytic principles and reaction mechanisms of enzymes.
  • Enzyme kinetics (steady-state and pre steady-state) and inhibition (reversible and irreversible). Transition-state inhibitor.
  • Binding energy and catalysis. Practical methods in enzymology, for example for studies of enzyme kinetics and reaction mechanisms, detection of intermediates, active-site titration.
  • Environmental effects on enzymes, for example pH, temperature, organic solvents.
  • Protein engineering, aims and strategies.

The course has many practical steps as in depth planning of experiments, information search emphasized on structures. Each student has to present a study on one enzyme in a written and oral report. The course ends with a written take-home examination.

Eligibility

Admission requirements for programme students at KTH:
At least 150 credits from grades 1, 2 and 3 of which at least 100 credits from years 1 and 2, and bachelor's work must be completed.  The 150 credits should include a minimum of 20 credits within the fields of Mathematics, Numerical Analysis and Computer Sciences, 5 of these must be within the fields of Numerical Analysis and Computer Sciences, 20 credits of Chemistry, possibly including courses in Chemical Measuring Techniques and 20 credits of Biotechnology or Molecular Biology.

Admission requirements for independent students:
20 ECTS in biochemistry, microbiology and gene technology/molecular biology; 20 ECTS in chemistry; 20 ECTS in mathematics/numerical analysis/computer science

Recommended prerequisites

KD1090 Organic chemistry 1, KD1100 Organic chemistry 2, BB1080/BB1090 Biochemistry or BB1050 Biotechnology for K.

Literature

Enzymes: A Practical Introduction to Structure, Mechanism, and data Analysis. Second edition. Robert A. Copeland. Wiley-VCH, ISBN 0-471-35929-7

Examination

  • INL1 - Literature Task, 1.5, grading scale: P, F
  • LAB1 - Laborator Work, 1.5, grading scale: P, F
  • TEN1 - Examination, 4.5, grading scale: A, B, C, D, E, FX, F

Requirements for final grade

Written take home examination (TEN1; 4,5 credits, grading scale A-F), Laboratory course (LAB1; 1,5 credits, grading scale Pass/Fail), Literature exercise (INL1; 1,5 credits, grading scale Pass/Fail).

Offered by

CBH/Industrial Biotechnology

Examiner

Pål Nyrén <nyren@kth.se>

Supplementary information

Students are required to sign up at least two weeks in advance for examination.

The course is given provided at least seven students are admitted.

Version

Course syllabus valid from: Autumn 2019.
Examination information valid from: Spring 2019.