The course contains 12 hours of lectures, 3 hours of seminars; and 14 hours of laboratory work including imaging, sample preparation and image processing, self study of material, one home assignment and oral presentation of the home assignment.
FCH3302 Cryo-Electron Microscopy Imaging and Analysis of Biological Materials 5.0 credits
Course on sample preparation and imaging of biological materials through electron cryo microscopy (cryoEM). The course explains the basic principle of image formation and image processing in transmission electron microscopy. The course is a theoretical and practical introduction into the technique of cryoEM. The goal is to familiarize the participants with the workflow in a cryoEM project, including image processing to obtain a 3D reconstruction. The technology is critically discussed to show the boundaries of this technology.
Information per course offering
Course offerings are missing for current or upcoming semesters.
Course syllabus as PDF
Please note: all information from the Course syllabus is available on this page in an accessible format.
Course syllabus FCH3302 (Spring 2021–)Headings with content from the Course syllabus FCH3302 (Spring 2021–) are denoted with an asterisk ( )
Content and learning outcomes
Course disposition
Course contents
The course is going to inform the attendants on the basic principles:
- Transmissionselektronmikroskopi och bildgivning
- Provberedning för kryoEM, material och kringutrustning
- Grunderna för och olika mjukvara för bildbearbetning and 3D- rekonstruktion.
Intended learning outcomes
After completion of the course the doctoralstudent should have the knowledge and ability to
- Explain the basic principles of image formation and image processing in cryoEM.
- Demonstrate adequate knowledge to implement and adopt sample preparation strategies for cryoEM
- Suggest, plan and implement a strategy for an own project suitable for cryoEM.
- Reflect critically on risks and environmental aspects on the sample preparation for yourself, co-workers and society.
- Present relevant studies and how they can improve your own research.
Literature and preparations
Specific prerequisites
No information inserted
Literature
You can find information about course literature either in the course memo for the course offering or in the course room in Canvas.
Examination and completion
If the course is discontinued, students may request to be examined during the following two academic years.
Grading scale
P, F
Examination
- LAB1 - Laboratory work, 3.0 credits, grading scale: P, F
- SEM1 - Seminars, 2.0 credits, grading scale: P, F
Based on recommendation from KTH’s coordinator for disabilities, the examiner will decide how to adapt an examination for students with documented disability.
The examiner may apply another examination format when re-examining individual students.
Presence in seminars and one approved oral presentation 15 min, laboratory work (in a group) and written project proposal (assignment).
Examiner
Ethical approach
- All members of a group are responsible for the group's work.
- In any assessment, every student shall honestly disclose any help received and sources used.
- In an oral assessment, every student shall be able to present and answer questions about the entire assignment and solution.
Further information
Course room in Canvas
Registered students find further information about the implementation of the course in the course room in Canvas. A link to the course room can be found under the tab Studies in the Personal menu at the start of the course.
Offered by
Main field of study
This course does not belong to any Main field of study.
Education cycle
Third cycle
Transitional regulations
If the course is discontinued, students may request to be examined during the following two academic years.