FCH3302 Cryo-Electron Microscopy Imaging and Analysis of Biological Materials 5.0 credits
Course on sample preparation and imaging of biological materials through electron cryo microscopy (cryoEM). The course explains the basic principle of image formation and image processing in transmission electron microscopy. The course is a theoretical and practical introduction into the technique of cryoEM. The goal is to familiarize the participants with the workflow in a cryoEM project, including image processing to obtain a 3D reconstruction. The technology is critically discussed to show the boundaries of this technology.
Information for research students about course offerings
Contact the examiner for information.
Content and learning outcomes
Course disposition
The course contains 12 hours of lectures, 3 hours of seminars; and 14 hours of laboratory work including imaging, sample preparation and image processing, self study of material, one home assignment and oral presentation of the home assignment.
Course contents
The course is going to inform the attendants on the basic principles:
- Transmissionselektronmikroskopi och bildgivning
- Provberedning för kryoEM, material och kringutrustning
- Grunderna för och olika mjukvara för bildbearbetning and 3D- rekonstruktion.
Intended learning outcomes
After completion of the course the doctoralstudent should have the knowledge and ability to
- Explain the basic principles of image formation and image processing in cryoEM.
- Demonstrate adequate knowledge to implement and adopt sample preparation strategies for cryoEM
- Suggest, plan and implement a strategy for an own project suitable for cryoEM.
- Reflect critically on risks and environmental aspects on the sample preparation for yourself, co-workers and society.
- Present relevant studies and how they can improve your own research.
Literature and preparations
Specific prerequisites
Recommended prerequisites
Equipment
Literature
Cryo-EM: A Unique Tool for the Visualization of Macromolecular Complexity:https://www.sciencedirect.com/science/article/pii/S1097276515001331
cryoSPARC Guide: https://cryosparc.com/docs
Cryo-EM reconstruction of the chlororibosome to 3.2 A˚ resolution within 24 h: https://journals.iucr.org/m/issues/2017/06/00/kf5004/kf5004.pdf
Examination and completion
If the course is discontinued, students may request to be examined during the following two academic years.
Grading scale
Examination
- LAB1 - Laboratory work, 3.0 credits, grading scale: P, F
- SEM1 - Seminars, 2.0 credits, grading scale: P, F
Based on recommendation from KTH’s coordinator for disabilities, the examiner will decide how to adapt an examination for students with documented disability.
The examiner may apply another examination format when re-examining individual students.
Presence in seminars and one approved oral presentation 15 min, laboratory work (in a group) and written project proposal (assignment).
Opportunity to complete the requirements via supplementary examination
Opportunity to raise an approved grade via renewed examination
Examiner
Ethical approach
- All members of a group are responsible for the group's work.
- In any assessment, every student shall honestly disclose any help received and sources used.
- In an oral assessment, every student shall be able to present and answer questions about the entire assignment and solution.
Further information
Course room in Canvas
Offered by
Main field of study
Education cycle
Add-on studies
Contact
Transitional regulations
If the course is discontinued, students may request to be examined during the following two academic years.