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FCH3302 Cryo-Electron Microscopy Imaging and Analysis of Biological Materials 5.0 credits

Course on sample preparation and imaging of biological materials through electron cryo microscopy (cryoEM). The course explains the basic principle of image formation and image processing in transmission electron microscopy. The course is a theoretical and practical introduction into the technique of cryoEM. The goal is to familiarize the participants with the workflow in a cryoEM project, including image processing to obtain a 3D reconstruction. The technology is critically discussed to show the boundaries of this technology.

Course offering missing for current semester as well as for previous and coming semesters
Headings with content from the Course syllabus FCH3302 (Spring 2021–) are denoted with an asterisk ( )

Content and learning outcomes

Course contents

The course is going to inform the attendants on the basic principles:

  • Transmissionselektronmikroskopi och bildgivning
  • Provberedning för kryoEM, material och kringutrustning
  • Grunderna för och olika mjukvara för bildbearbetning and 3D- rekonstruktion.

Intended learning outcomes

After completion of the course the doctoralstudent should have the knowledge and ability to

  • Explain the basic principles of image formation and image processing in cryoEM.
  • Demonstrate adequate knowledge to implement and adopt sample preparation strategies for cryoEM
  • Suggest, plan and implement a strategy for an own project suitable for cryoEM.
  • Reflect critically on risks and environmental aspects on the sample preparation for yourself, co-workers and society.
  • Present relevant studies and how they can improve your own research.

Course disposition

The course contains 12 hours of lectures, 3 hours of seminars; and 14 hours of laboratory work including imaging, sample preparation and image processing, self study of material, one home assignment and oral presentation of the home assignment.

Literature and preparations

Specific prerequisites

No information inserted

Recommended prerequisites

No information inserted

Equipment

No information inserted

Literature

Cryo-EM: A Unique Tool for the Visualization of Macromolecular Complexity:https://www.sciencedirect.com/science/article/pii/S1097276515001331

cryoSPARC Guide: https://cryosparc.com/docs

Cryo-EM reconstruction of the chlororibosome to 3.2 A˚ resolution within 24 h: https://journals.iucr.org/m/issues/2017/06/00/kf5004/kf5004.pdf

Examination and completion

If the course is discontinued, students may request to be examined during the following two academic years.

Grading scale

P, F

Examination

  • LAB1 - Laboratory work, 3.0 credits, grading scale: P, F
  • SEM1 - Seminars, 2.0 credits, grading scale: P, F
Based on recommendation from KTH’s coordinator for disabilities, the examiner will decide how to adapt an examination for students with documented disability.

The examiner may apply another examination format when re-examining individual students.

Presence in seminars and one approved oral presentation 15 min, laboratory work (in a group) and written project proposal (assignment).

Opportunity to complete the requirements via supplementary examination

No information inserted

Opportunity to raise an approved grade via renewed examination

No information inserted

Examiner

Ethical approach

  • All members of a group are responsible for the group's work.
  • In any assessment, every student shall honestly disclose any help received and sources used.
  • In an oral assessment, every student shall be able to present and answer questions about the entire assignment and solution.

Further information

Course web

Further information about the course can be found on the Course web at the link below. Information on the Course web will later be moved to this site.

Course web FCH3302

Offered by

Main field of study

This course does not belong to any Main field of study.

Education cycle

Third cycle

Add-on studies

No information inserted

Contact

Karsten Mim, Caroline Jegerschöld (cije@kth.se)

Transitional regulations

If the course is discontinued, students may request to be examined during the following two academic years.

Supplementary information

The lectures and the seminar will be zoom

The lab course will be in person

Postgraduate course

Postgraduate courses at CBH/Biomedical Engineering and Health Systems