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FCK3312 NMR Spectroscopy and imaging – quantum mechanical principles 4.5 credits

The course includes theory and practice for multidimensional NMR spectroscopy.

Course offering missing for current semester as well as for previous and coming semesters
Headings with content from the Course syllabus FCK3312 (Spring 2020–) are denoted with an asterisk ( )

Content and learning outcomes

Course contents

  • Single spin in magnetic field
  • The density operator formalism
  • The effect of nuclear spin interactions
  • Polarization transfer
  • The mechanism of 2D COSY and HSQC experiments
  • Dipole-dipole relaxation and the NOE effect
  • The mechanism of 2D NOESY experiment
  • Basic NMR strategies for biomolecular studies

Intended learning outcomes

After completing the course, the student should be able to:

  • explain the basic quantum mechanical principles for NMR spectroscopy
  • explain the operation of the most common multidimensional NMR experiments
  • explain how suitable combinations of NMR experiments can be used for structure determination of biomolecules
  • define and compare the different methods, choose technique/method for specific analysis problems and motivate the choices
  • use the above listed knowledge to plan, perform and evaluate a laboratory project within the subject area, in group (or individually, depending on the number of participants) including to perform a smaller literature study, and present the laboratory project in a written report and perform an opposition on another laboratory project in the course

Course disposition

The course comprises approximately 100 full-time study hours, whereof 30 hours Zoom lectures, in addition to self-studies. The lectures treat basic principles such as spin algebra, density operator formalism of quantum statistical mechanics, and the design and operation of the most common multidimensional NMR pulse sequences. The course also includes a short treatment of the application of those methods in structural biochemistry.

For participants without sufficient entry-level knowledge in quantum mechanics, a short additional introduction of  ”Quantum mechanics for pedestrians” will be given with the intention of facilitating the understanding of the actual course material.

Literature and preparations

Specific prerequisites

Knowledge corresponding to course FCK3311, NMR spectroscopy and imaging - basic principles, or equivalent.

Recommended prerequisites

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Understanding NMR Spectroscopy, J. Keeler, 2nd ed,

Distributed material.

Examination and completion

If the course is discontinued, students may request to be examined during the following two academic years.

Grading scale

P, F


  • LAB1 - Laboratory project, 1.5 credits, grading scale: P, F
  • RAP1 - Report, 3.0 credits, grading scale: P, F

Based on recommendation from KTH’s coordinator for disabilities, the examiner will decide how to adapt an examination for students with documented disability.

The examiner may apply another examination format when re-examining individual students.

Approved report (RAP1; 3 ECTS)

Approved presentation of the laboratory study (LAB1; 1.5 ECTS)

Other requirements for final grade

The student should be able to fulfil all parts of the objectives on both a general and basic level, and on a detailed level, and with both width and depth. The student should also be able to use this knowledge to set up multidimensional NMR experiments to gain insight to particular problems, and relate this to own research.

Opportunity to complete the requirements via supplementary examination

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Opportunity to raise an approved grade via renewed examination

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Ethical approach

  • All members of a group are responsible for the group's work.
  • In any assessment, every student shall honestly disclose any help received and sources used.
  • In an oral assessment, every student shall be able to present and answer questions about the entire assignment and solution.

Further information

Course web

Further information about the course can be found on the Course web at the link below. Information on the Course web will later be moved to this site.

Course web FCK3312

Offered by


Main field of study

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Education cycle

Third cycle

Add-on studies

No information inserted

Supplementary information

Not more than 30 participants. The course is to a certain extent carried out remotely via Zoom, the lectures (but not the lab) remain open to other participants.

Postgraduate course

Postgraduate courses at CBH/Chemistry