SK2520 Experimental Methods in Molecular Biophysics 8.0 credits

Experimentella metoder inom molekylär biofysik

The course gives an introduction to biomolecules, their structures, dynamics and interactions, and how experimental biophysical techniques can be used to investigate biomolecular properties. The course also gives an overview of how these techniques are used in practice within biotechnology, drug development, and academic research.

  • Education cycle

    Second cycle
  • Main field of study

    Biotechnology
    Engineering Physics
    Physics
  • Grading scale

    A, B, C, D, E, FX, F

Course offerings

Autumn 18 for Study Abroad Programme (SAP)

  • Periods

    Autumn 18 P1 (4.0 credits), P2 (4.0 credits)

  • Application code

    10010

  • Start date

    27/08/2018

  • End date

    14/01/2019

  • Language of instruction

    English

  • Campus

    AlbaNova

  • Tutoring time

    Daytime

  • Form of study

    Normal

  • Number of places

    No limitation

  • Schedule

    Schedule (new window)

  • Course responsible

    Jerker Widengren <jwideng@kth.se>

  • Target group

    Only SAP-students. Students from UCAS only.

    Physics Background

Autumn 18 for programme students

Autumn 19 for programme students

Intended learning outcomes

This course covers different experimental biophysical methods, how they are used to study structures and dynamics of biomolecules and their interactions. It also gives an overview of how these biophysical techniques are used in practice in biotechnology, drug development and in fundamental academic research. After this course the students are expected to be able to:

  • give a an overview of the main categories of biomolecules present in the human body, what their main functions are, and how they are built.
  • explain how interactions can take place between biomolecules and electromagnetic radiation
  • state what modern spectroscopic techniques that are used in molecular biophysics, and to explain the physical principles upon which these methods are based
  • Based on knowledge of the physical principles of the different biophysical techniques, judge and motivate which method(s) that is most appropriate to be applied to a particular biomolecular investigation.
  • Give an overview of how these methods are used in practice in biotechnology, drug development, clinical diagnostics and in fundamental academic research.

Course main content

Fundamental properties of biomolecules. Basic thermodynamics of biomolecules, biomolecular dynamics and interactions. The principles of the following methods: Infrared-, Fluorescence-, Nuclear Magnetic Resonance-, Electron Spin Resonance-, Circular Dichroism- and Raman-spectroscopy, Mass spectrometry, X-ray crystallography, Electron Microscopy, Surface Plasmon Resonance, Atomic Force Microscopy. An overview of applications of these techniques in fundamental academic research, in pharmaceutical and biotech industry, and for clinical diagnostics.

Lectures (34 h), laborations (8 h), study visit (6 h)

Eligibility

Achieved BSc on any of the programs CMATD, CDATE, CELTE, CTFYS, CMAST, CFATE, or
approved courses in mathematics and physics, corresponding to at least the courses in these subjects given on the above programs in the first three years.

Recommended previous knowledge:          
Fundamental knowledge in quantum mechanics and optics advantageous, but not absolutely required.

Literature

Nathan R. Zaccai, Igor N. Serdyuk, Joseph Zaccai. Methods in Molecular Biophysics Structure, Dynamics, Function for Biology and Medicine, 2nd Edition. Cambridge University Press

(aktuell upplaga anslås på kursens hemsida senast fyra veckor innan kursstart).

Vetenskapliga artiklar.

Laborationsinstruktioner.

Examination

  • LAB1 - Laboratory Work, 2.0, grading scale: P, F
  • PRO1 - Project, 1.0, grading scale: P, F
  • TEN1 - Examination, 5.0, grading scale: A, B, C, D, E, FX, F

One written examination (TEN1, 5hp, grades A-F), one oral project presentation (PRO1; 1hp, grades P/F), laborations, exercises and study visit (LAB1; 2hp, grades P/F)

Offered by

SCI/Applied undergraduate Physics

Contact

Jerker Widengren (jwideng@kth.se)

Examiner

Jerker Widengren <jwideng@kth.se>

Version

Course syllabus valid from: Autumn 2017.
Examination information valid from: Autumn 2007.