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SK2536 Laboratory techniques in Life Sciences 7.5 credits

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Headings with content from the Course syllabus SK2536 (Spring 2022–) are denoted with an asterisk ( )

Content and learning outcomes

Course contents

Cell culture laboratory: equipment, tools, consumables. Cell culture methods. Media and solutions. Contamination of cell cultures.

Measurement of cell viability. Good cell culture practice.

Electrophoresis, principles and applications for analysis of proteins in biological samples.

Quantification by densitometric methods.

PCR technology and applications in cell and molecular biology.

Labeling of cells for flow cytometry and microscope analysis with cytochemistry and immunocytochemistry.

Ethical and sustainability issues related to work in cell and molecular biology laboratory.

Intended learning outcomes

After completing the course, the student should be able to:

  • plan and perform cell culture, PCR, electrophoresis and labeling of cells for flow cytometry and microscope analysis.
  • plan, calculate and prepare solutions, e.g. cell culture medium.
  • describe the physical principles of PCR, electrophoresis and flow cytometry.
  • describe equipment, tools and consumables needed for cell culture, PCR, electrophoresis and cell labeling.
  • account for the different aspects of good practice when working in a cell and molecular biology laboratory.

Course disposition

No information inserted

Literature and preparations

Specific prerequisites

Completed course SK1104 Classical Physics or SK1114 Electromagnetism and waves.
Completed course SK1105 Experimental physics or HE1200 Electrical engineering and measurement technology.

English B / English 6

Recommended prerequisites

SK2532 (Biomedicine for Engineers) is recommended, but is not a requirement.

Equipment

No information inserted

Literature

No information inserted

Examination and completion

If the course is discontinued, students may request to be examined during the following two academic years.

Grading scale

A, B, C, D, E, FX, F

Examination

  • LAB1 - Laboratory work, 5.0 credits, grading scale: P, F
  • TEN1 - Written exam, 2.5 credits, grading scale: A, B, C, D, E, FX, F
Based on recommendation from KTH’s coordinator for disabilities, the examiner will decide how to adapt an examination for students with documented disability.

The examiner may apply another examination format when re-examining individual students.

Opportunity to complete the requirements via supplementary examination

No information inserted

Opportunity to raise an approved grade via renewed examination

Yes

Examiner

Ethical approach

  • All members of a group are responsible for the group's work.
  • In any assessment, every student shall honestly disclose any help received and sources used.
  • In an oral assessment, every student shall be able to present and answer questions about the entire assignment and solution.

Further information

Course web

Further information about the course can be found on the Course web at the link below. Information on the Course web will later be moved to this site.

Course web SK2536

Offered by

Main field of study

Engineering Physics

Education cycle

Second cycle

Add-on studies

No information inserted

Contact

Björn Önfelt (onfelt@kth.se), Marina Zelenina (marinaz@kth.se)

Supplementary information

Replaces SK2513.