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CB201V Biocatalysis for Industrial Professionals 4.0 hp

Course memo Spring 2022-20054

Version 1 – 01/07/2022, 2:38:52 PM

Course offering

Spring 2022-1 (Start date 18/01/2022, English)

Language Of Instruction

English

Offered By

CBH/Industrial Biotechnology

Course memo Spring 2022

Headings denoted with an asterisk ( * ) is retrieved from the course syllabus version Spring 2022

Content and learning outcomes

Course contents

The course is given online and remotely and provides an overview of the research area Biocatalysis, with special emphasis on the application of enzymes for environmentally sustainable production of chemical products. The course comprises approximately 105 full-time study hours, corresponding to 4,0 ECTS credits.

Relevant enzymes and their reaction mechanisms are discussed. Furthermore, central experimental techniques for the use of enzymes in organic solvents are discussed, as well as methods for optimizing stereochemical yields with industrial processes as examples.

Furthermore, the course provides knowledge about general and current techniques for design and modification of enzymes, for example directed evolution, for application in biocatalysis. The student carries out a project where a theoretical experiment is designed which includes, among other things, to select and justify the choice of methods for the experimental execution and the analysis of the results.

Intended learning outcomes

After completion of the course the student shall be able to:

  • demonstrate knowledge and analytical skills in the field of biocatalysis
  • demonstrate ability to explain and analyze concepts in biocatalysis based on relevant research literature 
  • formulate and discuss in writing how biocatalysis can be applied in industry to create more environmentally sustainable processes and, be able to reflect on sustainable societal development

Learning activities

The course contains a lecture series arranged jointly with the Masters' course BB2460 Biocatalysis. The lectures are mainly pre-recorded and the videos should be watched prior to each of the scheduled workshops, where discussions and problem-solving are the major activities. Apart from the workshops, the course contains an individual theoretical research project presented in a report. 

Detailed plan

Learning activities Content Preparations for workshop
Lecture 1 Introduction videos No scheduled workshop. Watch videos and read articles.
Lecture 2 Fine Chemicals Pre-recorded material, articles and slides
Lecture 3 Kinetic Resolution & Enantioselectivity Pre-recorded material, articles and slides
Lecture 4 Enzyme Mechanisms Pre-recorded material, articles and slides
Lecture 5 Hydrolases Pre-recorded material, articles and slides
Lecture 6 Oxidoreductases Pre-recorded material, articles and slides
Lecture 7 Transferases, Lyases, Isomerases & Ligases Pre-recorded material, articles and slides
Lecture 8 Organic Solvents & Water Activity Pre-recorded material, articles and slides
Lecture 9 Enzyme Engineering Strategies Pre-recorded material, articles and slides
Lecture 10 Immobilisation & Biocatalysis at a company Pre-recorded material, articles and slides
Lecture 11 Cascade Biocatalysis Pre-recorded material, articles and slides
Seminars The Master students present their Biocatalysis research projects Be an active participant and participate in the Q&A sessions


Schema VT-2022-976

Preparations before course start

Literature

The compulsory course literature consist of a few scientific review articles selected for each of the lectures in the course. These articles can be found through links provided for each lecture in Canvas. No compulsory text book is used but there are several optional books:

  • Kurt Faber. Biotransformations in Organic Chemistry:  A Textbook, 7th Edition. Springer-Verlag:  Heidelberg. 2018. e-ISBN 978-3-319-61590-5. Available as e-book through the KTH Library.
  • Andreas Liese, Karsten Seelbach, Christian Wandrey (Eds.). Industrial Biotransformations. 2nd ed. Wiley-VCH. 2006. e-ISBN: 9783527608188. DOI: 10.1002/3527608184. Available as e-book through the KTH Library.
  • Bommarius & Riebel, Biocatalysis - Fundamentals and Applications, Wiley-VCH 2004. e-ISBN: 9783527602360. Available as e-book through the KTH Library.
  • Silverman, R. B. The Organic Chemistry of Enzyme-Catalyzed Reactions, Academic Press. 2002. ISBN 978-0-08-051336-2. Available as e-book through the KTH Library.
  • Peter Grunwald, BIOCATALYSIS - Biochemical Fundamentals and Applications, Imperial College Press 2009. ISBN 978-1-86094-771-1 (used as course text book 2012)
  • Adrie J. J. Straathof, Patrick Adlercreutz (Eds.) Applied biocatalysis. 2nd Taylor & Francis 2000

Examination and completion

Grading scale

P, F

Examination

  • INL1 - Hand in assignments, 1.0 credits, Grading scale: P, F
  • PRO1 - Project, 3.0 credits, Grading scale: P, F

Based on recommendation from KTH’s coordinator for disabilities, the examiner will decide how to adapt an examination for students with documented disability.

The examiner may apply another examination format when re-examining individual students.

The section below is not retrieved from the course syllabus:

Hand in assignments ( INL1 )

This part is assessed through reflection tasks based on the precorded videos and submitted before each of the lectures and active participation in the scheduled workshops. Active participation in 80% of the workshops is required.

Project ( PRO1 )

This part is assessed by a written individual project report.

Ethical approach

  • All members of a group are responsible for the group's work.
  • In any assessment, every student shall honestly disclose any help received and sources used.
  • In an oral assessment, every student shall be able to present and answer questions about the entire assignment and solution.

Further information

No information inserted

Round Facts

Start date

18 Jan 2022

Course offering

  • Spring 2022-20054

Language Of Instruction

English

Offered By

CBH/Industrial Biotechnology

Contacts

Course Coordinator

Teachers

Examiner