Course on sample preparation and imaging of biological materials through electron cryo microscopy (cryoEM). The course explains the basic principle of image formation and image processing in transmission electron microscopy. The course is a theoretical and practical introduction into the technique of cryoEM. The goal is to familiarize the participants with the workflow in a cryoEM project, including image processing to obtain a 3D reconstruction. The technology is critically discussed to show the boundaries of this technology.
Course memo Spring 2021
Du hittar kurs-PM för nyare kursomgångar på sidan Kurs-PM.
Course presentation
Headings denoted with an asterisk ( * ) is retrieved from the course syllabus version Spring 2021
Content and learning outcomes
Course contents
The course is going to inform the attendants on the basic principles:
- Transmissionselektronmikroskopi och bildgivning
- Provberedning för kryoEM, material och kringutrustning
- Grunderna för och olika mjukvara för bildbearbetning and 3D- rekonstruktion.
Intended learning outcomes
After completion of the course the doctoralstudent should have the knowledge and ability to
- Explain the basic principles of image formation and image processing in cryoEM.
- Demonstrate adequate knowledge to implement and adopt sample preparation strategies for cryoEM
- Suggest, plan and implement a strategy for an own project suitable for cryoEM.
- Reflect critically on risks and environmental aspects on the sample preparation for yourself, co-workers and society.
- Present relevant studies and how they can improve your own research.
Learning activities
The course is going to inform the attendants on the basic principles of (english version of course content):
- Transmissionselectronmicroscopy and image formation
- Sample preparation for cryoEM, material och equipment
- The basics for and different software for image processing and 3D- reconstruction.
Detailed plan
| Learning activities | Content | Preparation |
|---|---|---|
| Zoom Lectures | Theoretical background | Literature recommended |
| On-site Practical microscopy |
Sample preparation and imaging. |
|
| On-site or remote practical |
Image processing |
Bring a laptop |
| Zoom Exam seminar day | Individual oral presentation of the written assignment: a cryoEM project proposal |
Preparations before course start
Literature
Cryo-EM: A Unique Tool for the Visualization of Macromolecular Complexity:https://www.sciencedirect.com/science/article/pii/S1097276515001331
cryoSPARC Guide: https://cryosparc.com/docs
Cryo-EM reconstruction of the chlororibosome to 3.2 A˚ resolution within 24 h: https://journals.iucr.org/m/issues/2017/06/00/kf5004/kf5004.pdf
Support for students with disabilities
Students at KTH with a permanent disability can get support during studies from Funka:
Examination and completion
Grading scale
P, F
Examination
- LAB1 - Laboratory work, 3.0 credits, Grading scale: P, F
- SEM1 - Seminars, 2.0 credits, Grading scale: P, F
Based on recommendation from KTH’s coordinator for disabilities, the examiner will decide how to adapt an examination for students with documented disability.
The examiner may apply another examination format when re-examining individual students.
Presence in seminars and one approved oral presentation 15 min, laboratory work (in a group) and written project proposal (assignment).
Ethical approach
- All members of a group are responsible for the group's work.
- In any assessment, every student shall honestly disclose any help received and sources used.
- In an oral assessment, every student shall be able to present and answer questions about the entire assignment and solution.
Further information
No information inserted
Contacts
Course Coordinator
Teachers
Examiner
Round Facts
Start date
31 May 2021
Course offering
- Spring 2021-61570
- Spring 2021-61573
Language Of Instruction
English