The course gives a theoretical and practical orientation of the methods used for purification of biomolecules (proteins, nucleic acid, metabolites etc.). General knowledge of the main steps in a purification process, from handling of biological samples and cell lysis, via the most common separation methods, to concentration and storage of final sample, is included. Basic experimental design will also be treated. The course contains both theoretical and practical parts.
The following methods will be covered in the course:
- Cell lysis
- Centrifugation
- Filtration
- Precipitation
- Chromatography (gel-filtration, ion exchange, hydrophobic interactions, affinity etc.)
- Buffer exchange, concentration and storage
After completion of the course the student shall have
Knowledge and understanding to:
- Account for, describe and give examples of common methods for purification of biomolecules. (TEN1)
- Apply methods for purification of biomolecules. (LAB1)
- Perform a chromatographic experiment for purification of a biomolecule, calculate the obtained yield and suggest improvements. (LAB1)
Skills and abilities to:
- Plan an efficient process for purification of a biomolecule (LAB1)
- Report orally and in writing within the subject (LAB1)
Values and approaches to
- Critically evaluate a presentation of an experiment and give constructive feedback. (LAB1)
For higher grades (A-D) the student shall also be able to
- Compare and pan complex experiments for purification of biomolecules. (TEN1)
