BB2255 Tillämpad genteknologi 7,5 hp

Applied Gene Technology

OBS!

Informationen nedan baseras på en kursplan som ännu inte har börjat gälla.

The course aims to give detailed insight into the techniques and trends in the fields of genomics and transcriptomics, DNA-assisted proteomics and high throughput data analysis in genomics and transcriptomics. This will build up the necessary foundations for further understanding of association studies, forensics, population genetics, diagnostics, medicine and drug development.

  • Utbildningsnivå

    Avancerad nivå
  • Huvudområde

    Bioteknik
  • Betygsskala

    A, B, C, D, E, FX, F

Kurstillfällen/kursomgångar

HT19 för programstuderande

HT18 för programstuderande

Lärandemål

Following completion and passing the course you should be able to:

  • Describe, illustrate and relate different techniques in the fields of genomics and transcriptomics
  • Critically evaluate, select and apply the most appropriate technique(s) in different biological and medical studies.
  • Discuss and suggest strategies to tackle and solve challenging problems in various research studies.
  • Construct and create biologically relevant studies by employing one or more of the discussed tools.
  • Explain the theory of state-of-the-art tools/algorithms for processing data from high-throughput molecular biology experiments in genomics and transcriptomics
  • Choose and use appropriate methods and tools for processing data from high-throughput molecular biology experiments in genomics and transcriptomics.

Kursens huvudsakliga innehåll

The course will focuses on describing, applying and relating state of the art technologies and high throughput data analysis.

Kursupplägg

The course includes a short introduction to conventional strategies for whole genome sequencing followed by description of different high throughput methods for typing of genetic variations, advanced techniques and platforms for DNA sequencing including whole genome sequencing, RNA-seq, single cell and single molecule transcript and protein (DNA-assisted) profiling.

The course also consists of a series of lectures and computer-based laboratory exercises including genome assembly, mapping of reads to reference genome, analysis of RNA-seq and ChIP-seq data.

In addition, the course involves a literature workshop of selected articles, which will be performed in groups. Each group presents one article and will oppose other groups’ articles. This project aims to teach critical reading, interpretation and comparison of the most advanced techniques and platforms in the fields of genome sequencing, massively parallel genotyping and single cell profiling. The project requires teamwork and planning, and participation in planning and execution of plans as well as presence on the workshop days is compulsory.

Behörighet

Litteratur

Distributed handouts and articles

Examination

  • PRO1 - Litteraturseminarium, 1,0, betygsskala: P, F
  • PRO2 - Datorlaboration, 2,5, betygsskala: P, F
  • TEN1 - Tentamen, 4,0, betygsskala: A, B, C, D, E, FX, F

Ges av

CBH/Genteknologi

Examinator

Afshin Ahmadian <afshin@kth.se>

Övrig information

The module ÖVN1 will not be used.

Versionsinformation

Kursplan gäller från och med HT2019.
Examinationsinformation gäller från och med VT2019.