FSK3515 Optimizing Light Microscopy 4.0 credits

The following elements are highlighted via seminars and exercises: Köhler illumination, conjugate plane, condenser, diffraction-limited resolution, aberrations, contrast through oblique illumination, phase contrast, Hoffman contrast, polarization microscopy, Nomarski / DIC, fluorescence and confocal microscopy.

The aim of the course is to give the students a deeper understanding of the optical principles behind different contrast-enhancing techniques used in modern research microscopes. The course will provide the students with practical skills enabling them to use microscopes for different studies and to make informed choices of appropriate contrast technologies based on the properties of the samples under investigation and the available functionalities of the microscope available.

The student should have been accepted as a PhD student

If the course is discontinued, students may request to be examined during the following two academic years.

• LAB1 - Laboratory work, 4.0 credits, grading scale: P, F

Based on recommendation from KTH’s coordinator for disabilities, the examiner will decide how to adapt an examination for students with documented disability.

The examiner may apply another examination format when re-examining individual students.

LAB1) laboratory tasks, 4.0 credits, grading scale: P/F

The examination consists of a practical knowlegde-test using the microscope.

Hjalmar Brismar

• All members of a group are responsible for the group's work.
• In any assessment, every student shall honestly disclose any help received and sources used.
• In an oral assessment, every student shall be able to present and answer questions about the entire assignment and solution.