The following elements are highlighted via seminars and exercises: Köhler illumination, conjugate plane, condenser, diffraction-limited resolution, aberrations, contrast through oblique illumination, phase contrast, Hoffman contrast, polarization microscopy, Nomarski / DIC, fluorescence and confocal microscopy.
FSK3515 Optimizing Light Microscopy 4.0 credits
Information per course offering
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Course syllabus as PDF
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Course syllabus FSK3515 (Autumn 2018–)Content and learning outcomes
Course contents
Intended learning outcomes
The aim of the course is to give the students a deeper understanding of the optical principles behind different contrast-enhancing techniques used in modern research microscopes. The course will provide the students with practical skills enabling them to use microscopes for different studies and to make informed choices of appropriate contrast technologies based on the properties of the samples under investigation and the available functionalities of the microscope available.
Literature and preparations
Specific prerequisites
The student should have been accepted as a PhD student
Recommended prerequisites
Equipment
Literature
Examination and completion
If the course is discontinued, students may request to be examined during the following two academic years.
Grading scale
Examination
- LAB1 - Laboratory work, 4.0 credits, grading scale: P, F
Based on recommendation from KTH’s coordinator for disabilities, the examiner will decide how to adapt an examination for students with documented disability.
The examiner may apply another examination format when re-examining individual students.
LAB1) laboratory tasks, 4.0 credits, grading scale: P/F
Other requirements for final grade
The examination consists of a practical knowlegde-test using the microscope.
Opportunity to complete the requirements via supplementary examination
Opportunity to raise an approved grade via renewed examination
Examiner
Ethical approach
- All members of a group are responsible for the group's work.
- In any assessment, every student shall honestly disclose any help received and sources used.
- In an oral assessment, every student shall be able to present and answer questions about the entire assignment and solution.