Course contents *

Models and equations describing the transport of water, ions and nutrients within cells and through cell membranes. Active and passive transport. Channels and carriers. Cell electrical potential (graded and action potential). Methods for measuring cellular physical parameters.

Lectures (24 hours), laboratory exercises (32 hours), hand-in assignment

Laboratory exercises:

• measurement of diffusion in an aqueous solution that mimics cytoplasm (fluorescent microscopy)
• measurement of diffusion in cell membranes (living cells, confocal microscopy)
• measurement of changes in cell volume (living cells, confocal microscopy)
• measurement of changes in cell membrane potential (living cells, confocal microscopy)

Intended learning outcomes *

Upon completion of the course, students should be able to:

• describe the basic physical principles of cell function in transport of water, ions and nutrients
• define mathematical models for transport of water, ions and solutes in and across the cell membrane
• describe how the cell regulates water and ion balance and how it is affected by the cell environment
• calculate the cell membrane potential and how it varies in different situations
• perform measurements of different cellular physical parameters using microscope-based methods

Course Disposition

No information inserted

Specific prerequisites *

Basic knowledge of mathematics (differential and integral calculus, linear algebra, differential equations) and physics (classical physics, electromagnetism).

Recommended prerequisites

Basic knowledge of mathematics (differential and integral calculus, linear algebra, differential equations) and physics (classical physics, electromagnetism).

Equipment

No information inserted

Literature

Weiss TF. Cellular Biophysics, vol 1: Transport, MIT Press, 1996

Weiss TF. Cellular Biophysics, vol 2: Electrical properties, MIT Press, 1997

If the course is discontinued, students may request to be examined during the following two academic years.

Grading scale *

A, B, C, D, E, FX, F

Examination *

• INL1 - Hand in assignment, 1.0 credits, Grading scale: P, F
• LAB1 - Laboratory experiments, 4.0 credits, Grading scale: P, F
• TEN1 - Written exam, 5.0 credits, Grading scale: A, B, C, D, E, FX, F

Based on recommendation from KTH’s coordinator for disabilities, the examiner will decide how to adapt an examination for students with documented disability.

The examiner may apply another examination format when re-examining individual students.

Other requirements for final grade *

Hand-in assignment (INL1 1,0 credits, grade scale: P, F)

Laboratory exercises (LAB1 4,0 credits, grade scale: P, F)

Written exam (TEN1 5,0 credits, grade scale: A, B, C, D, E, FX, F)

Opportunity to complete the requirements via supplementary examination

No information inserted

Opportunity to raise an approved grade via renewed examination

No information inserted

Examiner

Hjalmar Brismar

Ethical approach *

• All members of a group are responsible for the group's work.
• In any assessment, every student shall honestly disclose any help received and sources used.
• In an oral assessment, every student shall be able to present and answer questions about the entire assignment and solution.

Course web

Further information about the course can be found on the Course web at the link below. Information on the Course web will later be moved to this site.

Offered by

SCI/Applied Physics

Main field of study *

Biotechnology, Engineering Physics, Physics

Education cycle *

Second cycle

Add-on studies

No information inserted

Contact

Hjalmar Brismar (brismar@kth.se)

Supplementary information

Replaces SK2510 och SK2511