FSK3503 Cell Culture: Theory and Practice 4.0 credits

Cellodling: teori och praktik

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Course information

Content and learning outcomes

Course contents *

Purposes of cell culture. Safety and legislation. Cell culture laboratory: equipment, cleaning, appliances. Cell culture methods. Media and solutions. Freezing and thawing. Contamination of cell cultures. Methods for measuring cell viability / cell death.

Intended learning outcomes *

Upon completion of the course, students should be able to:

  • account for the basic characteristics of animal cells
  • account for the main needs of cultured cells / requirements for cell environment in cell culture
  • assess which equipment and consumables are needed for cell culture and for experiments on living cells
  • describe which modern techniques are used in cell culture
  • from the knowledge about the cell culture techniques and cell properties, be able to assess and motivate which cells and which cell culture techniques are most suitable for different experiments
  • perform the basic procedures in cell culture (count, replate cells, assess their condition)
  • understand the risks and ethical problems related to cell culture

Course Disposition

The course consists of 5 lectures, 3 laboratory exercises (4 hours each), and a seminar.

Laboratory exercises:
- replating of animal cells (two lab occasions)
- assessment of cell viability / cell death

Literature and preparations

Specific prerequisites *

Enrolled PhD student.

Recommended prerequisites

SK2530 (Introduction to Biomedicine) or SK2531 (Biomedicine for Engineers) are strongly recommended.


No information inserted


Lecture notes, cell culture protocol.
ECACC Handbook  - Fundamental Techniques for ECACC Cell Lines (online).

Examination and completion

Grading scale *

P, F

Examination *

  • LAB1 - Laboratory exercises, 2.0 credits, Grading scale: P, F
  • SEM1 - Seminar, 0.5 credits, Grading scale: P, F
  • TEN1 - Written exam, 1.5 credits, Grading scale: P, F

Based on recommendation from KTH’s coordinator for disabilities, the examiner will decide how to adapt an examination for students with documented disability.

The examiner may apply another examination format when re-examining individual students.

Other requirements for final grade *

Written exam (TEN1 1.5 credits, grade scale: P, F)
Laboratory exercises (LAB1 2.0 credits, grade scale: P, F)
Seminar (SEM1 0.5 credits, grade scale: P, F)

Opportunity to complete the requirements via supplementary examination

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Opportunity to raise an approved grade via renewed examination

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Hjalmar Brismar

Further information

Course web

Further information about the course can be found on the Course web at the link below. Information on the Course web will later be moved to this site.

Course web FSK3503

Offered by

SCI/Applied Physics

Main field of study *

No information inserted

Education cycle *

Third cycle

Add-on studies

No information inserted


Marina Zelenina, marinaz@kth.se

Ethical approach *

  • All members of a group are responsible for the group's work.
  • In any assessment, every student shall honestly disclose any help received and sources used.
  • In an oral assessment, every student shall be able to present and answer questions about the entire assignment and solution.

Supplementary information

The number of participants is limited.

Postgraduate course

Postgraduate courses at SCI/Applied Physics