FSK3503 Cell Culture: Theory and Practice 4.0 credits
This course has been discontinued.
Last planned examination: Spring 2023
Decision to discontinue this course:
No information insertedContent and learning outcomes
Course contents
Purposes of cell culture. Safety and legislation. Cell culture laboratory: equipment, cleaning, appliances. Cell culture methods. Media and solutions. Freezing and thawing. Contamination of cell cultures. Methods for measuring cell viability / cell death.
Intended learning outcomes
Upon completion of the course, students should be able to:
- account for the basic characteristics of animal cells
- account for the main needs of cultured cells / requirements for cell environment in cell culture
- assess which equipment and consumables are needed for cell culture and for experiments on living cells
- describe which modern techniques are used in cell culture
- from the knowledge about the cell culture techniques and cell properties, be able to assess and motivate which cells and which cell culture techniques are most suitable for different experiments
- perform the basic procedures in cell culture (count, replate cells, assess their condition)
- understand the risks and ethical problems related to cell culture
Literature and preparations
Specific prerequisites
Enrolled PhD student.
Recommended prerequisites
SK2530 (Introduction to Biomedicine) or SK2531 (Biomedicine for Engineers) are strongly recommended.
Equipment
Literature
Lecture notes, cell culture protocol.
ECACC Handbook - Fundamental Techniques for ECACC Cell Lines (online).
Examination and completion
If the course is discontinued, students may request to be examined during the following two academic years.
Grading scale
Examination
- LAB1 - Laboratory exercises, 2.0 credits, grading scale: P, F
- SEM1 - Seminar, 0.5 credits, grading scale: P, F
- TEN1 - Written exam, 1.5 credits, grading scale: P, F
Based on recommendation from KTH’s coordinator for disabilities, the examiner will decide how to adapt an examination for students with documented disability.
The examiner may apply another examination format when re-examining individual students.
Other requirements for final grade
Written exam (TEN1 1.5 credits, grade scale: P, F)
Laboratory exercises (LAB1 2.0 credits, grade scale: P, F)
Seminar (SEM1 0.5 credits, grade scale: P, F)
Opportunity to complete the requirements via supplementary examination
Opportunity to raise an approved grade via renewed examination
Examiner
Ethical approach
- All members of a group are responsible for the group's work.
- In any assessment, every student shall honestly disclose any help received and sources used.
- In an oral assessment, every student shall be able to present and answer questions about the entire assignment and solution.
Further information
Course room in Canvas
Offered by
Main field of study
Education cycle
Add-on studies
Contact
Supplementary information
The number of participants is limited.