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Per Olofsson

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About me

MSc. (Civ.ing.) Engineering Physics (KTH, 2011).

Önfelt Lab PhD student in Biological Physics: December 2011–January 2017.

Research interests: NK cell biology, single-cell analysis, single-cell imaging, 3-D NK cell functional assays, functional heterogeneity of NK cells, immune cell migration

ORCID ID:0000-0002-6019-8157.

Journal publications:

  1. Forslund E, Guldevall K, Olofsson PE, Frisk T, Christakou AE, Wiklund M and Önfelt B (2012) Novel microchip-based tools facilitating live cell imaging and assessment of functional heterogeneity within NK cell populations. Front. Immunol. 3:300. DOI:10.3389/fimmu.2012.00300. DiVA:diva2:610341.
  2. Vanherberghen B, Olofsson PE, Forslund E, Sternberg-Simon M, Khorshidi MA, Pacouret S, Guldevall K, Enqvist M, Malmberg KJ, Mehr R and Önfelt B. (2013) Classification of human natural killer cells based on migration behavior and cytotoxic response. Blood. 121:1326-34. DOI:10.1182/blood-2012-06-439851. DiVA:diva2:640782.
  3. Olofsson PE, Forslund E, Vanherberghen B, Chechet K, Mickelin O, Ahlin AR, Everhorn T, Önfelt B. (2014) Distinct Migration and Contact Dynamics of Resting and IL-2-Activated Human Natural Killer Cells. Front. Immunol. 5:451. DOI:10.3389/fimmu.2014.00080. DiVA:diva2:723531. Erratum: DOI:10.3389/fimmu.2014.00451.
  4. Forslund E, Sohlberg E, Enqvist M, Olofsson PE, Malmberg KJ and Önfelt B. (2015) Microchip-Based Single-Cell Imaging Reveals That CD56dimCD57-KIR-NKG2A+ NK Cells Have More Dynamic Migration Associated with Increased Target Cell Conjugation and Probability of Killing Compared to CD56dimCD57-KIR-NKG2A- NK Cells. J.Immunol.195:3374-3381. DOI:10.4049/jimmunol.1500171. DiVA:diva2:865375.
  5. Guldevall K, Brandt L, Forslund E, Olofsson K, Frisk T, Olofsson PE, Gustafsson K, Manneberg O, Vanherberghen B, Brismar H, Kärre K, Uhlin M and Önfelt B. (2016) Microchip Screening Platform for Single Cell Assessment of NK Cell Cytotoxicity. Front. Immunol.7:119. DOI:10.3389/fimmu.2016.00119. DiVA:diva2:924247.
  6. Olofsson PE*, Brandt L*, Magnusson KEG, Frisk T, Jaldén J and Önfelt B. (2019) A collagen-based microwell migration assay to study NK—target cell interactions. Sci Rep: 9:10672. DOI:10.1038/s41598-019-46958-3. DiVA:diva2:1341937. *shared first authorship
  7. Verron Q, Forslund E, Brandt L, Leino M, Frisk TW, Olofsson PE and Önfelt B. (2021) NK cells integrate signals over large areas when building immune synapses but require local stimuli for degranulation. Accepted Sci. Signal. bioRxiv DOI:doi.org/10.1126/scisignal.abe2740.

Other publications:

  1. Olofsson P (2011) Microwell-based Collagen Matrix Migration Assays for NK:target Cell Interactions: Three-dimensional Imaging and Analysis of Transient Migration Behavior of NK Cellsin vitro. Master thesis. KTH Royal Institute of Technology. URN:urn:nbn:se:kth:diva-43456. DiVA:diva2:448517.
  2. Olofsson P (2014) Quantitative approaches to studying NK cell functional heterogeneity. Licentiate thesis. KTH Royal Institute of Technology. ISBN: 978-91-7595-166-9. URN:urn:nbn:se:kth:diva-146281. DiVA:diva2:723534.
  3. Vanherberghen B, Frisk T, Forslund E, Olofsson PE, Guldevall K and Önfelt B. (2016) Microwell-Based Live Cell Imaging of NK Cell Dynamics to Assess Heterogeneity in Motility and Cytotoxic Response. In: Somanchi SS (ed)Natural Killer Cells: Methods and Protocols. Humana Press, New York. ISBN: 978-1-4939-3682-3. DOI:10.1007/978-1-4939-3684-7_8.
  4. Olofsson PE (2016) Microscopy-based single-cellin vitro assays for NK cell function in 2-D and 3-D. Doctoral thesis. KTH Royal Institute of Technology. ISBN: 978-91-7729-241-8. URN:urn:nbn:se:kth:diva-199571.DiVA:diva2:1062979.

Conference contributions:

  1. Olofsson P, Frisk T and Önfelt B. (2012) Time-lapse Imaging of NK Cell Migration in 3‐D Collagen Matrices Formed in Microwells, 13th Meeting of the Society for Natural Immunity, April 20–24 (Abstract)
  2. Olofsson PE, Forslund E, Vanherberghen B and Önfelt B. (2014) Comparison of contact dynamics of resting and IL-2-activated human natural killer cells, Biophysical Society 58th Annual Meeting, February 15–19 (Abstract)
  3. Olofsson PE, Forslund E and Önfelt B (2014) Quantitative single-cell approaches to NK cell functional heterogeneity, Single Cell Genomics 2014, September 9–11 (Abstract)
  4. Olofsson P, Frisk T and Önfelt B. (2016) Microwell-based collagen-matrix migration assays to study NK-target interactions. 16th Meeting of the Society for Natural Immunity, October 2-5 (Abstract)

Teaching and departmental duties

I have helped teach the following courses: Cellular Biophysics I (SK2510), Electromagnetism and Waves (SK1110/..,), Classical Physics (SK1102), Physics of Biomedical Microscopy (SK2500). In addition, I have co-supervised two bachelor thesis projects in Engineering Physics (DiVA:diva2:642343,DiVA:diva2:651356)

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