Silk proteins have a unique propensity to assemble their chains in an ingenious way so that a material with favourable properties is formed.
We have surveyed the silk assembly mechanism, and are investigating how to best control it to achieve desired formats, e.g. fibers, coatings and fibrillar networks.
By the use of biotechnology methods, it is feasible to combine various functional molecules. We have developed methods to functionalize silk either at genetic or protein level. This allows us to design and construct fibrillar networks with incorporated functionalities such as specific affinity, enzymatic activity, antimicrobial effects and cell stimulation.
The main focus of my current research is to utilise silk assembly in combination with biotechnology methods to mimic the in vivo environment, i.e. the extracellular matrix (ECM), to create functional tissue for biomedical applications. For example, we develop functional in vitro models of cancer, to be used for evaluation of drug candidates as well as pre-tests to allow personalized treatment plans.
Mona Widhe, Ronnie Jansson, Caterina Collodet, Chris Tasiopoulos, Danilo Hirabae de Olivieira, Kelly Blust, Nicolai Dorka, Linnea Gustafsson (MST)
Johansson U, Widhe M, Shalaly ND, Arregui IL, Nilebäck L, Tasiopoulos CP, Åstrand C, Berggren PO, Gasser C, Hedhammar M
Assembly of functionalized silk together with cells to obtain proliferative 3D cultures integrated in a network of ECM-like microfibers.
Sci Rep. 2019 Apr 18;9(1):6291
Thatikonda, N., Nilebäck, L., Kempe, A., Widhe, M., Hedhammar, M
Bioactivation of Spider Silk with Basic Fibroblast Growth Factor for in Vitro Cell Culture: A Step toward Creation of Artificial ECM
ACS Biomaterials Science and Engineering, 2018 4 (9), pp 3384–3396
Tasiopoulos CP, Widhe, Hedhammar M
Recombinant spider silk functionalized with a motif from fibronectin mediates cell adhesion and growth on polymeric substrates by entrapping cells during self-assembly.
ACS Applied Materials & Interfaces, 2018 May 2;10(17):14531-14539.
Gustafsson L, Jansson R, Hedhammar M, van der Wijngaart W c.
Structuring of Functional Spider Silk Wires, Coatings, and Sheets by Self-Assembly on Superhydrophobic Pillar Surfaces.
Adv Mater. 2018 Jan;30(3).
Nilebäck L, Arola S, Kvick M, Paananen A, Linder MB, Hedhammar M
Interfacial Behavior of Recombinant Spider Silk Protein Parts Reveals Cues on the Silk Assembly Mechanism.
Langmuir. 2018 Oct 2;34(39):11795-11805.
Mittal N, Jansson R, Widhe M, Benselfelt T, Håkansson KMO, Lundell F, Hedhammar M, Söderberg LD
Ultrastrong and Bioactive Nanostructured Bio-Based Composites.
ACS Nano. 2017 May 23;11(5):5148-5159.
Nilebäck L, Hedin J, Widhe M, Floderus LS, Krona A, Bysell H, Hedhammar M
Self-Assembly of Recombinant Silk as a Strategy for Chemical-Free Formation of Bioactive Coatings: A Real-Time Study.
Biomacromolecules. 2017 Mar 13;18(3):846-854
Shalaly ND, Ria M, Johansson U, Åvall K, Berggren PO, Hedhammar M
Silk matrices promote formation of insulin-secreting islet-like clusters.
Biomaterials. 2016 Jun;90:50-61.
Widhe M, Shalaly ND, Hedhammar M
A fibronectin mimetic motif improves integrin mediated cell biding to recombinant spider silk matrices.
Biomaterials. 2016 Jan;74:256-66
Jansson R, Courtin CM, Sandgren M c, Hedhammar M
Rational Design of Spider Silk Materials Genetically Fused with an Enzyme
Advanced Functional Materials 2015 July; 25(33) 5343-52
Jansson R, Thatikonda N, Lindberg D, Rising A, Johansson J, Nygren PÅ, Hedhammar M
Recombinant spider silk genetically functionalized with affinity domains.
Biomacromolecules. 2014 May 12;15(5):1696-706
Renberg B, Andersson-Svahn H, Hedhammar M
Mimicking silk spinning in a microchip.
Sensors and Actuators B: Chemical. 2014;195: 404–408
Müller C, Hamedi M, Karlsson R, Jansson R, Marcilla R, Hedhammar M , Inganäs O c
Woven Electrochemical Transistors on Silk Fibers.
Advanced Materials. 2011;23(7):898-901.
Askarieh G*, Hedhammar M*, Nordling K, Saenz A, Casals C, Rising A, Johansson J , Knight SD
Self-assembly of spider silk proteins is controlled by a pH-sensitive relay
Hedhammar M, Bramfeldt H, Baris T, Widhe M, Askarieh G, Nordling K, Aulock S, Johansson J
Sterilized recombinant spider silk fibers of low pyrogenicity.
Biomacromolecules. 2010; 11(4):953-9.