Technology seminar

Instructions

The student seminar on high-throughput technologies aims to make you learn the ideas and characteristics of common and well known high-throughput experimental technologies. Instead of having a teacher explain the methods, we ask you to do self-studies of these technologies and prepare so that you can give a quick presentation of these methods. You are expected to know these methods to such a level that you can explain it to a peer.

At the seminar, we will pick random students that are asked to present the technologies (one each) that are listed below. The presentation should take about 5 minutes and you can use the board if you like. No computer presentations or other presentation materials are allowed. We have a quick and simple common discussion after each presentation. 

The lecture is mandatory and we expect active participation. If you are unable to attend or if it is clear that you have not learnt or prepared anything about the sessions topics, you will be asked to write a short essay describing the listed technologies. 

What to present?

You should explain, roughly, how the technologies work and what the data characteristics are: how much data is generated? What kind of errors and how many are typically made? Finally, you should conclude your presentation by mentioning a scientific article based on data from the presented technology.

When?

Lecture 4, 2016-01-27 at 10-12 in FA31.

Topics

DNA sequencing

• Paired ends and mate pair sequencing on the Illumina platform
• Single Molecule Sequencing (PacBio/Oxford Nanopore)

Transcriptomics

• Affymetrix microarrays
• RNA-Seq

Proteomics

• Shotgun proteomics
• Stable Isotope Labeling (SILAC)
• Selected reaction monitoring