Postdoc in spatial proteomics

Job description

The research group headed by Emma Lundberg is connected to KTH and located at SciLifeLab in Stockholm. Our research is focused on cell biology, spatial proteiomics and machine learning for bioimage analysis. The aim is to understand how human proteins are distributed in time and space, how this affects various cellular processes, and how changes in protein localization may contribute to disease processes. 

The group is responsible for the subcellular part of the Human Protein Atlas (HPA); a large research program based in Sweden that been systematically mapping the localization of human proteins in cells, tissues and organs during the past 20 years. For this project, we map the subcellular distribution of human proteins in cell lines using immunofluorescence (ICC-IF) and confocal microscopy. The research group is also applying the latest methods for highly multiplexed immunostaining of cells and tissues, and develops various tools for bioimage analysis, mostly using machine learning and AI-based models.

As a postdoc you will conduct research using various methods in cell-and molecular biology, but mainly cell culture, immunostaining od cells and tissues, confocal microscopy, and bioimage analysis. You will be involved in setting up a protocol for automated optical pooled screening (OPS) in the research group and use this method in your own research project. This project will revolve around the expression and distribution of metabolic enzymes, in close collaboration with the HPA team.

We are looking for someone with good collaborative- and communicational skills. At the same time, we prefer that you can work independently and take initiative. As a person, you are thorough and skilled in documenting your work accordingly, with an analytical mindset.

Qualifications

Requirements

• A doctoral degree or an equivalent foreign degree in in biotechnology, cell biology, molecular biology, biomedicine or a similar subject. This eligibility requirement must be met no later than the time the employment decision is made.
• Good theoretical knowledge in cell- and molecular biology.
• Experience of laboratory work using cell- and molecular biology methods.
• Excellent knowledge in English, both in terms of speaking and writing, as it is the language used for communication in the research group, as well as for scientific presentations and publications.
• Good collaborative skills that can be applied in a multidisciplined research team.
• Good communication skills.
• The ability to work independently and take initiatives in order to progress research projects.

Preferred qualifications

• A doctoral degree or an equivalent foreign degree, obtained within the last three years prior to the application deadline
• Experience in cell culture, particularly of human cell lines and hiPSCs.
• Experience in immunostaining of cells and/or tissues.
• Experience in confocal fluorescence microscopy.
• Experience in using liquid handling robots.
• Experience in bioimage analysis, for example using CellProfiler, R Bioconductor, ImageJ, and ML models.
• Experience in genetic (CRISPR) screening.
• Ability work efficiently and thourough.
• Good analytical skills.
• Awareness of diversity and equal opportunity issues, with specific focus on gender equality

Great emphasis will be placed on personal skills.

Join us at KTH

KTH shapes the future through education, research and innovation. As a leading international technical university, we play an active role in advancing the transition towards a sustainable society. At KTH, you have the opportunity to grow and develop in a creative and dynamic environment, with good working conditions and attractive benefits. Equality, diversity and equal opportunities are essential to quality and form an integral part of KTH’s core values as a university and public authority.

Learn more about our benefits and what it's like to work and grow at KTH.

Trade union representatives

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To apply for the position

Log into KTH's recruitment system to apply for this position. You are responsible for ensuring that your application is complete according to the instructions in the ad.

The application must include:

• CV including relevant professional experience and knowledge.
• Copy of diplomas and grades from your previous university studies. Translations into English or Swedish if the original documents have not been issued in any of these languages.
• Brief account of why you want to conduct research, your academic interests and how they relate to your previous studies and future goals. Maximally two pages long.

Your complete application must be received at KTH no later than the last day of application, midnight CET/CEST (Central European Time/Central European Summer Time).

About the employment

The position offered is for, at the most, three years.

A position as a postdoctoral fellow is a time-limited qualified appointment focusing mainly on research, intended as a first career step after a dissertation.

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Disclaimer: In case of discrepancy between the Swedish original and the English translation of the job announcement, the Swedish version takes precedence.