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Welcome to the course in Proteomics

Learning outcomes

The aim of the course is to provide the students with an introduction to current methodologies and trends in the field of proteomics. The student should also obtain an overview and awareness of typical proteomics applications. After completed course the student should be able to:

  • describe and discuss the possibilities and advantages, and the complexity and drawbacks of various proteomics technologies
  • compare traditional methods with emerging technologies
  • suggest suitable approaches for specified applications and motivate the choice
  • speculate and argue about the future of proteomics technologies
  • participate in scientific discussions regarding proteomics technologies
  • critically evaluate scientific results

Course main content

The course is focused on different methods, technologies and strategies currently used within the field of proteomics in general and with an emphasis on biomarker discovery. The lectures will cover background and recent advances for both classical proteomics methods, such as 2D-gel electrophoresis and mass spectrometry, and strategies based on high-throughput antibody generation, bioinformatics and structural approaches.


  • Knowledge corresponding to the following courses, or similar, is required.
  • BB1010 Introduction to Biotechnology
  • BB1020 Cell biology with Immunology
  • BB1080 Biochemistry, Theory
  • BB1110 Gene Technology and Molecular Biology
  • BB1130 Analysis and Purification of Biomolecules 


The examination consists of two parts:

  • The oral exam will be based on understanding and presenting a summary of aspects within proteomics. This will be a group activity, where each groups will prepare slides to be presented to the class based on a given subject. Each group will describe the content and discuss the information, drawbacks and limitations in relation to proteomics methods and approaches. It is important to see this part as not only an examination, but also as a learning activity and preparation for the written exam.
  • The written exam consists of discussion type questions, where you are expected to show that you have obtained the knowledge according to the aims of the course. You will also be able to express your opinion regarding various aspects of proteomics technologies. Opinions cannot be right or wrong but the way the argumentation is done will reveal your knowledge.

Requirements for final grade

  • An oral examination (1 hp, Pass/Fail)
  • A written examination (5 hp, grading scale A-F).

Reading material

  • Handouts: Provided at each lecture and available on Bilda
  • Course book: Principles of Proteomics by R.M Twyman, Garland Science, ISBN: 9780815344728 (second edition)

Course coordinator and Examiner

Jochen Schwenk


T: +46 8 5248 1482

Science for Life Laboratory | Division of Proteomics and Nanobiotechnology | School of Biotechnology | Tomtebodavägen 23A | SE-171 65 Solna


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