Good to be back to ESACT2022 conference

So good to be back to ESACT2022 conference! Great conference! AdBIOPRO hosted a workshop about perfusion process, co-chaired by Véronique Chotteau, Yvonne Genzel from Max Plank Inst. and Nandita Vishwanathan, Merck-Serono, with high participation both form industry and academia. Also great to see our achievements at AdBIOPRO and iConsensus presented in many oral and poster communications.

Oral presentations

OVERCOMING PRODUCT RETENTION IN TFF-BASED PERFUSION CELL

CULTURES BY USING LOW SHEAR MAGNETICALLY LEVITATING PUMPS

Magdalena Pappenreiter¹, Hubert Schwarz², Bernhard Sissolak³, Alois Jungbauer⁴,

Véronique Chotteau².

¹Bilfinger Life Science GmbH, Innovation Management; University of Natural Resources

and Life Sciences (BOKU), Vienna, Austria; ²KTH Royal Institute of Technology, Cell

Technology Group; AdBIOPRO, Competence Centre for Advance BioProduction by

Continuous Processing, Stockholm, Sweden; ³Bilfinger Life Science GmbH, Innovation

Management, Vienna, Austria; ⁴University of Natural Resources and Life Sciences (BOKU),

Vienna, Austria.

KNOWING MORE FROM LESS: A MICROFLUIDIC TOOLBOX FOR AT-LINE

MONITORING OF BIOPROCESSES

Inês Pinto¹, Leila Josefsson², Saara Mikkonen³, Debbie Van Der Burg³, Fabien Abeille⁴,

David Sergeant⁵, Meeri Mäkinen³, Sebastian Giehring⁶, Matthias Stich⁷, Christian

Krause⁷, Cari S.nger², Åsa Emmer³, Aman Russom¹, Véronique Chotteau³.

¹Science for Life Laboratory & KTH Royal Institute of Technology, Stockholm, Sweden;

²Kantisto, Baarn, Netherlands; ³ KTH & AdBIOPRO;

⁴Micronit, Enschede, Netherlands; ⁵IPRATECH, Mons, Belgium; ⁶PAIA Biotech, Köln,

Germany; ⁷Presens Precision Sensing, Regensburg, Germany.

Poster Student Highlights

RAMAN SPECTROSCOPY FOR MONITORING OF AMINO ACIDS AND

ANTIBODY GLYCOSYLATION IN HIGH CELL DENSITY PERFUSION CULTURE

Hubert Schwarz¹, Meeri M.kinen¹, Andreas Castan², Véronique Chotteau¹.

¹Competence Centre for Advanced BioProduction by Continuous Processing

(AdBIOPRO), Royal Institute of Technology (KTH), Cell Technology Group, Stockholm,

Sweden; ²Cytiva, Uppsala, Sweden.

Posters

FEEDING STRATEGY DESIGN FOR CONTROL OF IGG GLYCOSYLATION IN CHO

CELL PERFUSION CULTURES*

Liang Zhang¹, Hubert Schwarz¹, Andreas Castan², Håkan Hjalmarsson¹, Véronique

Chotteau¹.

¹KTH Royal Institute of Technology, Stockholm, Sweden; ²Cytiva Life Science, Uppsala,

Sweden.

ANTIBODY CAPTURE BASED ON MAGNETIC BEADS FROM CULTURE AT

DENSITY > 100 X 1E6 CELLS/ML*

Nils Brechmann¹, Hubert Schwarz¹, Kristofer Eriksson², Véronique Chotteau¹.

¹KTH, Royal Institute of Technology, Stockholm, Sweden; ²MAGic Bioprocessing, Uppsala,

Sweden.

PROOF-OF-CONCEPT OF A NOVEL CELL SEPARATION TECHNOLOGY USING

MAGNETIC AGAROSE-BASED BEADS

Nils Brechmann¹, Kristofer Eriksson², Véronique Chotteau¹.

¹KTH, Royal Institute of Technology, Stockholm, Sweden; ²MAGic Bioprocessing, Uppsala,

Sweden.

TRANSCRIPTOMICS AND MODELLING TO UNDERSTAND THE BENEFITS OF

LOW PERFUSION RATE*

Meeri Mäkinen¹, Hubert Schwarz¹, Magnus Lundqvist², Johan Rockberg², Véronique

Chotteau¹.

¹KTH, AdBIOPRO, Cell Technology Group, Sweden; ²KTH, AdBIOPRO, Protein Science,

Sweden.

HIGH CELL DENSITY PERFUSION PROCESS FOR RAAV PRODUCTION BASED

ON TRANSIENT TRANSFECTION

Ye Zhang, Véronique Chotteau, Emil Sundäng Peters.

AdBIOPRO, VINNOVA Competence Centre for Advanced BioProduction by Continuous

Processing, KTH Royal Institute of Technology and Cell Technology Group (CETEG), Dept.

of Industrial Biotechnology, School of Engineering Sciences in Chemistry, Biotechnology

and Health, KTH, Stockholm, Sweden.

THE EFFECT OF CELL DENSITY ON THE PLASMID UTILIZATION FOR

THE PRODUCTION OF ADENO-ASSOCIATED VIRUS VIA THE TRIPLETRANSFECTION

METHOD

Brian Ladd¹, Andreas Castan², Torbjörn Gräslund¹, Véronique Chotteau¹.

¹KTH, Stockholm, Sweden; ²Cytiva, Uppsala, Sweden.

STRATEGY FOR PERFUSION MEDIUM OPTIMIZATION IN MICRO-SCALE ERBI

BIOREACTORS COMBINED WITH DESIGN OF EXPERIMENTS METHODOLOGY*

Hubert Schwarz¹, Andreas Castan², Véronique Chotteau¹.

¹Competence Centre for Advanced BioProduction by Continuous Processing

(AdBIOPRO), Royal Institute of Technology (KTH), Cell Technology Group, Stockholm,

Sweden; ²Cytiva, Uppsala, Sweden.

INTEGRATED CONTINUOUS BIOMANUFACTURING AT PILOT-SCALE SUITABLE

FOR PH-SENSITIVE MONOCLONAL ANTIBODIES*

Véronique Chotteau¹, Hubert Schwarz¹, Joaquín Gomis Fons², Madel.ne Isaksson²,

Julia Scheffel¹, Andreas Castan³, Sophia Hober¹, Bernt Nilsson².

¹KTH Royal Institute of Technology, Stockholm, Sweden; ²Lund University, Lund, Sweden;

³Cytiva Life Science, Uppsala, Sweden.

Workshops

HOW TO DEVELOP PERFUSION PROCESSES?

Chairs: Véronique Chotteau (AdBIOPRO and KTH), Yvonne Genzel (Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany), Nandita Vishwanathan (Merck-Serono, Switzerland)

• Development of perfusion processes, Veronique Chotteau.

• Integrated continuous USP-DSP from small-scale to pilot-scale, Hubert Schwarz, KTH.

• Industrial perspective on monitoring and control of perfusion processes, Nandita Vishwanathan and Thomas Vuillemin, Merk.

Transcriptomics as a tool to understand perfusion processes, Meeri Mäkinen, AdBIOPRO and KTH.

• Evaluating end-to-end continuous bioprocesses from economic, environmental and PAT

perspectives, Catarina Pereira Galo Neves, UCL.

• An integrated perfusion process for cell culture-based production of MVA virus for vaccination and gene therapy, Sven Göbel, Max Planck Institute for Dynamics of Complex Technical Systems

Presentation in workshop FROM RESEARCH TO CLINIC: MODERN APPROACHES TO BIOPHARMA COMMERCIALIZATION, moderator: Andreas Castan, Director Strategic Technologies, Cytiva

• Continuous biopharma production processes, Véronique Chotteau, AdBIOPRO and KTH

Presentation in workshop ANALYTICAL STRATEGIES FOR IMPROVED PRODUCT CHARACTERIZATION & PROCESS UNDERSTANDING, organized by ACTIP – Animal Cell Technology Industrial Platform

• iConsensus IMI project creating a sensing platform for a biopharmaceutical

cultivation process and high-throughput system, Véronique Chotteau, Coordinator of iConsensus, AdBIOPRO and KTH.

*selected top 100