The Protein Factory/The Tegel Lab
The focus of the Protein Factory is to produce and purify proteins in a high-throughput workflow. Full-length proteins are produced in mammalian cells within the Secretome project (Wallenberg Center for Protein Research) and for the Human Protein Atlasproject antigens are produced and polyclonal antibodies are purified.
Staff
Anna Berling (Team leader)
Gabriella Jensen (Research engineer)
Daniella Hamnqvist (Research engineer)
Our Research
The overall aim of the Human Secretome Project is to produce, purify and characterize the majority of all human secreted proteins to build up a resource of reagents for drug discovery and development. A high-throughput protein production workflow, using mammalian cells as host cells, has been set up and to purify the produced proteins a mild and effective one-step affinity purification based on affinity chromatography has been developed. The setup used within the Human Secretome Project is also used for protein production within CellNova , a VINNOVA centre for development and production of next generation biological drugs.
The Human Protein Atlas is a Swedish-based program with the aim to map all the human proteins in cells, tissues and organs. To be able to do that polyclonal antibodies are used. For that purpose, we produce and purify antigens and purify the generated polyclonal antisera.
Since 2022 the Protein Factory is also the locla node of Protein Production Sweden (PPS) at KTH. PPS is a national distributed research infrastructure established with funding from the Swedish Research Council, focused on production and purification of protein reagents on-demand. The aim is to serve Swedish researchers in academia as well as industry. The local node of PPS at KTH is focusing on protein production in mammalian cells.