Mathias Uhlén

Dr. Mathias Uhlén, Professor of Microbiology.

Mathias Uhlén

KTH Biotechnology
AlbaNova University Center
Royal Institute of Technology (KTH)
106 91 Stockholm, Sweden

Direct phone: +46 8 790 99 93
Secr: +46 8 790 99 87



Short biography

Dr Uhlen received his PhD at the Royal Institute of Technology (KTH), Stockholm, Sweden in 1984.  After a post-doc period at the EMBL in Heidelberg, Germany, he became professor in microbiology at KTH in 1988. His research is focused on protein science, antibody engineering and precision medicine and range from basic research in human and microbial biology to more applied research, including clinical applications in cancer, infectious diseases, cardiovascular diseases, autoimmune diseases and neurobiology. The research has resulted in more than 550 publications with a current h-index of 98 (Google Scholar). His group was the first to describe a number of innovations in science including:

  • engineered protein A and protein G for purification of antibodies1,2
  • affinity tags for purification of recombinant fusion proteins3
  • solid phase methods for DNA handling using the biotin-streptavidin system4
  • Pyrosequencing leading to the first next generation DNA sequencing instrument (454/Roche)5
  • Affibodies – protein binders aimed for therapeutic applications6

Since 2003, he has led an international effort to systematically map the human proteome with antibodies and to create an open source knowledge-based resource called the Human Protein Atlas7,8,9  ( He is member of the National Academy of Engineering (NAE) in USA, the Royal Swedish Academy of Science (KVA), the Swedish Academy of Engineering Science (IVA) and the European Molecular Biology Organization (EMBO). He is the President of the European Federation of Biotechnology and he is chairing both the Scientific Advisory Board of the UniProt consortium and the International Working Group for Antibody Validation (IWGAV)10.. From 2010-2015, he was the founding Director of the Science for LIfe Laboratory (SciLifelab) which is a national center for molecular bioscience  (

Key references:

  1. Uhlen et al (1984) J Biol Chem 259: 1695-702
  2. Guss et al (1988) EMBO J 5: 1567-75
  3. Nilsson, Abrahmsen and Uhlen (1985) EMBO J 4: 1075-80
  4. Uhlen (1989) Nature 340: 733-4
  5. Ronaghi, Uhlen and Nyren (1998) Science 281: 363-5
  6. Nord et al (1997) Nature Biotechnology 15: 772-7
  7. Uhlen et al (2010) Nature Biotechnology 28: 1248-50
  8. Uhlen et al (2015) Science 347: 1260419
  9. Uhlen et al (2016) Mol Systems Biol., 4;12:862
  10. Uhlen et al (2016) Nature Methods, in press.

Scientific summary (pdf 124 kB)

Research: High-throughput Proteomics and Functional Genomics

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